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Portal > Offres > Offre UMR7277-THILEP-002 - Identification d'ARN localisés dans les embryons précoces et des types cellulaires qui dépendent de Nodal, FGF et BMP (H/F)

Identification of RNAs localized in early embryos and cell types that depend on Nodal, FGF and BMP (M/F)

This offer is available in the following languages:
- Français-- Anglais

Application Deadline : 28 October 2025 23:59:00 Paris time

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General information

Offer title : Identification of RNAs localized in early embryos and cell types that depend on Nodal, FGF and BMP (M/F) (H/F)
Reference : UMR7277-THILEP-002
Number of position : 1
Workplace : NICE
Date of publication : 07 October 2025
Type of Contract : Researcher in FTC
Contract Period : 30 months
Expected date of employment : 1 November 2025
Proportion of work : Full Time
Remuneration : The remuneration will be based on experience and will range from €3,021.50 to €4,208.37 gross.
Desired level of education : Doctorate
Experience required : 1 to 4 years
Section(s) CN : 22 - Cellular biology, development, evolution-development, reproduction

Missions

The first part of the project is the identification of mRNAs asymmetrically distributed in the
different blastomeres of the early embryo. A few mRNAs including the maternally expressed transcript coding
for the TGF-beta antagonist Panda, and the zygotically expressed mRNA coding for the TGF-beta Nodal have
been identified but systematic searches for early localized mRNAs have not been done and it is not known whether
in addition to panda and nodal, other early asymmetries of localized maternal mRNA or of zygotic genes exist and
if they are involved in axis specification. One goal of the PhD project will be to identify maternal/early zygotic
transcripts asymmetrically distributed in blastomeres of the 4-, 8-, 16-, 32- and 60-cell stage embryos by
using single cell RNA-sequencing. This sc-RNA-seq approach is ideally suited to identify early localized mRNA,
potential maternal determinants of the D/V axis.
The second goal of the project is to identify the cell types that depend on Nodal, BMP or FGF to be specified.
Although cell types are key building blocks of multicellular organisms, and although Nodal, BMPs and FGFs are
key signals that instruct cells during development, no detailed description of the cell types specified by Nodal,
Production of synchronous embryo cultures at different embryonic stages of wild embryos or embryos treated with pharmacological agents, dissociation of blastomeres at different stages, passage on the chrome apparatus and collection of samples after reverse transcription.

Activities

Production of synchronous embryo cultures at different embryonic stages of wild embryos or embryos treated with pharmacological agents, dissociation of blastomeres at different stages, passage on the chrome apparatus and collection of samples after reverse transcription.

Skills

manipulation of sea urchin embryos (gamete collection, fertilization, filtration, determination of embryonic stage, dissociation). Analysis of the spatial expression of transcripts identified by in situ hybridization. Functional analyses by gain and loss of function. Construction of genetic networks.

Work Context

The Valrose Institute of Biology (IBV) is an international research institute comprising 27 teams and 250 members. It has three supervisory bodies: CNRS, Inserm, and Université Côte d'Azur. The institute is located in Nice on the campus of the Faculty of Sciences. The team is made up of four people.

Constraints and risks

Adapting schedules to experimental protocols