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Reference : UMR7277-ROBARK-009
Workplace : NICE
Date of publication : Monday, October 11, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 December 2021
Proportion of work : Full time
Remuneration : The salary is between 2690 and 3821 Euros, gross monthly depending on experience.
Desired level of education : PhD
Experience required : Indifferent
The candidate will take advantage of an optogenetic system, that has been recently established in Candida albicans, to study the dynamics of cell polarity in this opportunistic human pathogenic fungus, using a variety of molecular and cellular approaches.
A 1-2 year Post-Doc fellowship is available in the Polarized growth in yeast group at the Institute of Biology Valrose (iBV) at the University of Côte d'Azur in Nice, FR
Our team is studying the spatial and temporal regulation of polarized growth. Our goal is to understand the mechanisms of polarized growth, cellular morphogenesis and development in two model yeasts, the baker's yeast Saccharomyces cerevisiae and the opportunistic human pathogenic yeast, Candida albicans. The objective of this project is to quantitatively determine the repercussions of a spatio-temporal perturbation of polarity in two types of highly asymmetric cells. We will focus on the pear-shaped cells of S. cerevisiae (shmoo), induced in the presence of pheromone, and the hyphal filamentous cells of C. albicans. To study the temporal and spatial control of cell polarity in these yeast, which are different sizes and shapes, light-dependent systems (optogenetics) will be used to recruit an active form of Cdc42 to the plasma membrane. We will determine how this recruitment, site-specific or uniform, modifies the growth and the shape of the cells, the membrane compartments, the organization of the cytoskeleton, the distribution of polarity proteins and that of membrane lipids. Applicants with training in cell biology and biochemistry are encouraged to apply and contact Robert Arkowitz (firstname.lastname@example.org).
1) C Puerner, A Serrano, RS Wakade, M Bassilana & RA Arkowitz. A myosin light chain is critical for fungal growth robustness in Candida albicans. mBio. 2021. 12: e02528-21.
2) R Abdul-Ganiyu, LA Venegas, X Wang, C Puerner, RA Arkowitz, BK Kay & DE Stone. Phosphorylated Gbeta is a directional cue during yeast gradient tracking. Science Signaling. 2021. 14: eabf4710
3) C Puerner, N Kukhaleishvili, D Thomson, S Schaub, X Noblin, S Seminara, M Bassilana & RA Arkowitz. Mechanical force-induced morphology changes in a human fungal pathogen. BMC Biology. 2020. 18: 122.
4) M Bassilana, C Puerner & RA Arkowitz. External signal mediated polarized growth in fungi. Curr Op Cell Biol. 2020 62:150-158.
5) PM Silva, C Puerner, A Seminara, M Bassilana & RA Arkowitz. Secretory vesicle clustering in fungal filamentous cells does not require directional growth. Cell Reports. 2019 28: 2231–2245.
6) RA Arkowitz & M Bassilana. Recent advances in understanding Candida albicans hyphal growth. F1000 Research. 2019 8.
7) A Weiner, F Orange, S Lacas-Gervais, K Rechav, V Ghugtyal, M Bassilana & RA Arkowitz. On-site secretory vesicle delivery drives filamentous growth in the fungal pathogen Candida albicans. Cellular Microbiology. 2019 21: e12963.
8) H Labbaoui, S Bogliolo, V Ghugtyal, NV Solis, SG Filler, RA Arkowitz & M Bassilana. Role of Arf GTPases in fungal morphogenesis and virulence. PLoS Pathogens. 2017 13: e1006205.
9) V Ghugtyal, R Garcia-Rodas, A Seminara, S Schaub, M Bassilana & RA Arkowitz. A Phosphatidylinositol-4-phosphate-dependent membrane traffic is critical for fungal filamentous growth. PNAS USA. 2015 112: 8644.
10) SG Martin & RA Arkowitz. Cell polarization in budding and fission yeasts. FEMS Microbiology Review. 2014 38: 228
11) V Corvest, S Bogliolo, P Follette, RA Arkowitz & M Bassilana. Spatiotemporal regulation of Rho1 and Cdc42 activity during Candida albicans filamentous growth. Molecular Microbiology. 2013 89: 626.
12) I Guillas, A Vernay, JJ Vitagliano & RA Arkowitz. Phosphatidylinositol 4,5-bisphosphate is required for invasive growth in Saccharomyces cerevisiae. Journal Cell Science. 2013 126: 3602.
13) A Vernay, S Schaub, I Guillas, M Bassilana & RA Arkowitz. A steep phosphoinositide-bis-phosphate gradient forms during fungal filamentous growth. J Cell Biol. 2012 198: 711.
- An experience with fungal cells is a plus but is not necessary.
- Experience in microscopy.
- Sense of initiative and ability to work independently.
- Ability to work in a team and to communicate.
- Ability to carry out careful controlled and rigorous experiments.
- Sense of organization and autonomy.
- Ease of communication in written and oral English.
The Valrose Institute of Biology (27 teams; 300 people; 25 nationalities) is an international institute (English is the working language) with a very rich scientific environment. The iBV provides cutting-edge technological infrastructures and platforms, with a collaborative and lively atmosphere in a very attractive city / region. (ibv.unice.fr)
The recruitment starting date can be after the 1st of December.
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