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Reference : UMR7277-ROBARK-003
Workplace : NICE
Date of publication : Friday, February 28, 2020
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 May 2020
Proportion of work : Full time
Remuneration : between 2600 and 3000 gross monthly, depending on experience.
Desired level of education : PhD
Experience required : 1 to 4 years
The candidate will take advantage of an optogenetic system, that has been recently established in Candida albicans, to study the dynamics of cell polarity in this opportunistic human pathogenic fungus, using a variety of molecular and cellular approaches.
A 2-year Post-Doc fellowship is available in the Polarized growth in yeast group at the Institute of Biology Valrose (iBV) at the University of Côte d'Azur in Nice, FR
Our team is studying the spatial and temporal regulation of polarized growth. Our goal is to understand the mechanisms of polarized growth, cellular morphogenesis and development in two model yeasts, the baker's yeast Saccharomyces cerevisiae and the opportunistic human pathogenic yeast, Candida albicans. The objective of this project is to quantitatively determine the repercussions of a spatio-temporal perturbation of polarity in two types of highly asymmetric cells. We will focus on the pear-shaped cells of S. cerevisiae (shmoo), induced in the presence of pheromone, and the hyphal filamentous cells of C. albicans. To study the temporal and spatial control of cell polarity in these yeast, which are different sizes and shapes, light-dependent systems (optogenetics) will be used to recruit an active form of Cdc42 to the plasma membrane. We will determine how this recruitment, site-specific or uniform, modifies the growth and the shape of the cells, the membrane compartments, the organization of the cytoskeleton, the distribution of polarity proteins and that of membrane lipids. Applicants with training in cell biology and biochemistry are encouraged to apply and contact Robert Arkowitz (firstname.lastname@example.org).
1. M. Bassilana, C. Puerner & R. A. Arkowitz. Curr Op Cell Biol. 2019 62:150.
2. R. A. Arkowitz & Bassilana M. F1000 Res. 2019 8.
3. P. M. Silva, C. Puerner, A. Seminara, M. Bassilana & R. A. Arkowitz. Cell Rep. 2019 28: 2231.
4. A. Weiner, F. Orange, S. Lacas-Gervais, K. Rechav, V. Ghugtyal, M. Bassilana & R. A. Arkowitz. Cell Microbiol. 2019 21: e12963.
5. H. Labbaoui, S. Bogliolo, V. Ghugtyal, N. V. Solis, S. G. Filler, R. A. Arkowitz & M. Bassilana. PLoS Pathogens. 2017 13: e1006205.
6. V. Ghugtyal, R. Garcia-Rodas, A. Seminara, S. Schaub, M. Bassilana & R. A. Arkowitz. PNAS USA. 2015 112: 8644.
7. S. G. Martin & R. A. Arkowitz. FEMS Microbiol Rev. 2014 38: 228
8. V. Corvest, S. Bogliolo, P. Follette, R. A. Arkowitz & M. Bassilana. Mol. Microbiol. 2013 89: 626.
9. I. Guillas, A. Vernay, J. J. Vitagliano & R. A. Arkowitz. J. Cell Sci. 2013 126: 3602.
10. A. Vernay, S. Schaub, I. Guillas, M. Bassilana & R. A. Arkowitz. J Cell Biol. 2012 198: 711.
- An experience with fungal cells is a plus but is not necessary.
- Experience in microscopy.
- Sense of initiative and ability to work independently.
- Ability to work in a team and to communicate.
- Ability to carry out careful controlled and rigorous experiments.
- Sense of organization and autonomy.
- Ease of communication in written and oral English.
The Valrose Institute of Biology (27 teams; 300 people; 25 nationalities) is an international institute (English is the working language) with a very rich scientific environment. The iBV provides cutting-edge technological infrastructures and platforms, with a collaborative and lively atmosphere in a very attractive city / region. (ibv.unice.fr)
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