Postdoctoral Researcher in Animal Developmental Biology (M/F)

Job Description

Missions

The goal of this postdoctoral project is to determine the role of transcription factors in the sex determination process in zebrafish. The postdoctoral fellow will study the impact of genetic mutations in candidate genes involved in gonadal development using zebrafish models. Part of the project involves handling zebrafish embryos, maintaining lines via PCR genotyping, performing immunohistochemical and 3D imaging analyses of developing gonads, and conducting single-cell transcriptomic analyses (scRNA-seq) to understand the pathophysiological mechanisms underlying variations in genital development.

Activity

- Plan and conduct experimental studies - Manage colonies of transgenic zebrafish and identify them through genotyping - Collect and dissect juvenile and adult zebrafish, perform thin sections using a microtome and cryostat - Implement and adapt the various immunohistochemistry techniques required for the genetic analysis of zebrafish models, including histological (3D imaging) and molecular phenotyping - Use specialized software for imaging (ImageJ, AxioVision, etc.) and statistics (GraphPad) - Analyze “omics” data - Analyze and communicate data (maintain lab notebooks, present results at progress meetings) - Maintain a strong literature review - Potentially supervise a Master's student - Write a research article in English - Ensure compliance with health and safety principles and regulations.

Your Profil

Skills

-Ph.D. with training in reproduction or developmental biology and two to seven years of postdoctoral experience
-Extensive knowledge of fish anatomy and sexual differentiation processes
-Expertise in fish genetics, cellular and molecular biology, and microscopy
-Extensive experience in animal experimentation (a designer-level license to conduct experiments on live animals is desirable),
-Knowledge of imaging, histology (staining, microscopy), and FACS
-Proficiency in Word, Excel, PowerPoint, GraphPad, and image analysis software (Image, Photoshop, etc.).
-Organizational skills, planning ability, creativity, professionalism, motivation, perseverance, autonomy, and attention to detail
-Ability to work independently while also being adaptable and a team player
-Oral and written communication in English (equivalent to B2 level)

Your Work Environment

The team is located in the heart of Nice and is part of the Valrose Institute of Biology (iBV) (27 teams; 250 people; 25 nationalities), an international research center that brings together top-tier teams with complementary expertise and a shared interest in translating basic research into clinical knowledge. The iBV provides state-of-the-art facilities, with a collaborative and vibrant atmosphere in a very pleasant city and region.

Constraints and risks

The position involves handling zebrafish. Extensive experience in animal experimentation is required.

Compensation and benefits

Compensation

between €3,800 and €4,500 gross per month

Annual leave and RTT

44 jours

Remote Working practice and compensation

Pratique et indemnisation du TT

Transport

Prise en charge à 75% du coût et forfait mobilité durable jusqu’à 300€

About the offer

About the CNRS

The CNRS is a major player in fundamental research on a global scale. The CNRS is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to bring together different disciplines to address the most important challenges of the contemporary world, in connection with the actors of change.

CNRS

The research professions