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Portail > Offres > Offre UMR7277-ARNHUB-005 - Post-doctorat en biologie cellulaire, H/F

Post-doctorate in Cell Biology, H/F

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : lundi 13 février 2023

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General information

Reference : UMR7277-ARNHUB-005
Nombre de Postes : 1
Workplace : NICE
Date of publication : Monday, January 23, 2023
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 March 2023
Proportion of work : Full time
Remuneration : between 2833.40 € and 3257.06 € depending on the candidate expertise
Desired level of education : PhD
Experience required : 1 to 4 years

Missions

The researcher will coordinate the research project entitled « RNA condensate remodeling during oocyte growth » that will be conducted in the epitranscriptomics team of the intitute Biology Valrose

Activities

The researcher will coordinate the following project:
Spatiotemporal control of maternal mRNAs in early development is critical for cell identity specification. Decades of research have focused on characterizing maternal mRNAs and their consequent protein products in oogenesis and embryogenesis, with less experimental attention dedicated to the biophysics of supramolecular assemblies that mRNAs are packaged into and their dynamic remodelling. Our findings strikingly uncovered that C. elegans maternal mRNAs are stored in ribonucleoprotein condensates that are dynamically remodelled throughout oogenesis. While some mRNAs are sequentially released from condensates to be translated, others are stored and protected for later embryonic development. Here, we propose (1) to systematically identify the remodelled interactions controlling RNA condensate compositional changes during oocyte growth, (2) to dissect how microtubules and RNA polymers regulate condensate supramolecular assembly, dynamics, and material properties, and (3) test how condensate phase separations protect mRNAs from decay. The researcher will utilize our recently developed Fluorescent Activated Particle Sorting method to characterize the transcriptome and proteome of RNA condensates at distinct oocyte growth stages. Structural nodes, multivalent interactions, RNA repeats, and regulators such as microtubules will be manipulated to test their impact on mRNA stability, condensate assembly, remodelling, and physical properties (i.e. viscoelasticity, cluster size, partition coefficient, saturation concentrations). This work would represent the first comprehensive assessment of RNA condensate compositional dynamics and their impact on RNA expression and stability in a cell specification model. Together,
these studies will bring paradigm-shifts in our understanding of gene expression control, and reveal the emerging functions of a higher-scale of transcriptome organization that is conserved throughout evolution, from nematodes to mammals.

Skills

-Expertise in Cell Biology, Molecular Biology Biochemistry, developmental biology
-Expertise to develop bio-computing code for image analysis
-Autonomy to plan, conduct and analyse experiments
-Scientific article writing skills
-Oral communication
-Team spirit
-Training ability (the researcher will train students)
-Health and safety rules
-Scientific ethics

Work Context

The work will be conducted in the epitranscriptomics team under Arnaud Hustenberger supervision
http://ibv.unice.fr/research-team/hubstenberger/

Constraints and risks

-Applying health and safety rules
-Maintaining C. elegans strains
-Manipulate chemicals such as Formaldehyde, Tryzol, Phenol, Chloroforme, Sodium Azide, Methanol, Formamide

Additional Information

none

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