Missions

Postdoctoral position on the mechanisms of replication of a damaged DNA, mutagenesis and lesion tolerance in the bacteria Escherichia coli.

Activities

-construction of yeast strains
-construction of plasmid substrates containing a single lesion
-genetic experiments
-proteomic analysis
-structural analysis of proteins-proteins interactions
-Development of techniques for mapping single-stranded DNA gaps
-writing articles
-possibility of teaching (Master's degree)

Skills

-PhD in molecular biology (with or without postdoctoral experience)
-Experience in DNA replication and repair.
-Experience with Escherichia coli (not essential, but recommended)

Work Context

The team "DNA damage and genome instability" led by Dr. Vincent Pagès at CRCM aims to understand the mechanisms of replication of damaged DNA, as well as the genetics of DNA damage tolerance pathways.
The team has developed a method to introduce a single lesion in the genome of a living cell (bacteria and yeast), allowing them to monitor the fate of this lesion in vivo.
The proposed postdoctoral research project will explore the genetic and molecular mechanisms of DNA damage tolerance pathways in E. coli. We will identify new factors involved in lesion tolerance pathways by pulling down the damaged region using a dead-Cas9 system. We will then characterize the role of these factors and identify their partners by using a combination of computational analyses, single molecule imaging and molecular approaches such as Electron Microscopy. The project will be realized in collaboration with research groups at Harvard Medical School (Boston – USA) and Institut Gustave Roussy (Paris – France).