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Portail > Offres > Offre UMR7256-ESTGRO-015 - Ingénieur-e H/F en caractérisation structurale et fonctionnelle de la machinerie d'assemblage du génome des Mimiviridae

researcher H/F Structural and functional characterization of the machinery used by the Mimiviridae to compact their genomes

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : mardi 2 novembre 2021

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General information

Reference : UMR7256-ESTGRO-015
Workplace : MARSEILLE 09
Date of publication : Tuesday, October 12, 2021
Type of Contract : FTC Technical / Administrative
Contract Period : 12 months
Expected date of employment : 1 December 2021
Proportion of work : Full time
Remuneration : Monthly Salary between 2750 and 3840 € depending on experience
Desired level of education : Engineer
Experience required : Indifferent

Missions

The IGS laboratory is offering a Post-doctoral position in the context of and ERC grant. The present position will aim to elucidate how the Mimiviridae genome is packaged inside the icosahedral capsids. As the disorganized DNA is packaged through at least one lipidic membrane into a rod-shaped helicoidal structure with a 30nm section, a specific assembly machinery must be devoted to the genome packaging. The ultimate goal of the project will be to compare this machinery with the DNA translocation machineries used in the cellular and the viral world.

Activities

A first objective of the project will be to extract the membranes of the purified capsids in order to identify the membrane proteins (viral and cellular) which compose it. We are currently working on the expression of an enzyme involved in the loading and compaction of viral DNA conserved in all major nucleocytoplasmic DNA viruses. This enzyme will be used as a bait to identify membrane proteins capable of interacting in order to study them structurally and functionally, in vitro and in infected cells. The candidate will express the identified proteins and use standard methodologies (nanodisks, crystallization, Cryo-EM) to resolve the structures of individual proteins and pre-assembled complexes in vitro.

Skills

- PhD in Biological Science or Physics
- Strong expertise in structural biology of membrane proteins: biochemistry, CryoEM and Crystallography.
- Any citizenship
- Constrains: Data acquisition at synchrotron are made in the context of a shared Bag which means that the applicant will have to spend time on the beamlines collecting data for the IGS but also for other members of the BAG. Missions are not frequent but can take place during nights and week-end.

Work Context

The IGS laboratory is a pioneer and a world leader in the study of giant viruses for which we discovered the four known families. A unique strength of the IGS laboratory is the coexistence of a computational biology team and an experimental biology team, allowing efficient and seamless continuity from isolation of new microorganisms to their comprehensive characterization using genomics, transcriptomics and proteomics. The laboratory also has the capacity to determine the 3D-structure of viral proteins of specific interest using X-ray crystallography alone or in combination with Electron Microscopy. An ERC advanced grant starting in October 2019 provides two post-doctoral positions and two PhD grants to study the structural organization of the genomes of the Mimiviridae and identify the machinery responsible for their packaging into rod shaped structures filling the 450nm icosahedral virions.

Constraints and risks

Constrains: Data acquisition at synchrotron are made in the context of a shared Bag which means that the applicant will have to spend time on the beamlines collecting data for the IGS but also for other members of the BAG. Missions are not frequent but can take place during nights and week-end

Additional Information

To apply for the Position at IGS, the candidate must send his/her CV with a complete list of publications, a motivation letter and at least one reference letter at https://bit.ly/3lghz08

The selected candidate can start 2021. The position is for 1 year and can be renewed for 3 years.

The IGS laboratory is already well equipped for cellular biology (2 L2 rooms, 6 PSM, 3 fluorescence microscopes (including 1 Zeiss Axio), 4 incubators), biochemistry (PCR, 1 Microbiology PSM, 3 incubators, 2 Aktä explorer 10S, 1 Aktä 3D, 1 Aktä Prime, Nanodrop, Qubit, 96-well and 1mL spectrophotometers), and structural biology (Mosquito crystallization robot, in-house diffractometer (Oxford diffraction Xcalibur PXUltra, a speedvac SPD131 ThermoFischer). The IGS has direct access to the 12 technical platforms at the Mediterranean Institute of Microbiology (IMM, AMU-CNRS FR3479, Staff: 300). The platforms of interest for the project are fluorescence/TEM imaging (TEM, Cryo-TEM), NMR (600 MHz, cryoprobe), Proteomics, protein expression and protein interaction facility (1 Aktä prime, 1 Aktä Pure). The protein production platform runs a Blitz system used to rapidly screen and characterize macromolecular interactions. The IGS also has permanent access to the Luminy campus imagery platforms including electron microscopy (TEM and STEM) at the IBDM (Institut de Biologie du Développement de Marseille). The IGS masters all the required skills in cellular biology (light, fluorescence and electron microscopy, virus purification, synchronized infections, extraction, and molecular studies), structural biology, biochemistry and virology methods. The laboratory is also part of the Marseille synchrotron BAG and has access to European synchrotron radiation facilities and Soleil for crystallography. The Bag also provides free of charge access to the EM platforms at ESRF equipped with Titan Krios cryo-electron microscope with K2 Summit direct electron detector, a Quantum LS imaging filter and a Volta phase plate (VPP). This microscope will be run as a beamline (CM01) within the Partnership for Structural Biology (PSB) together with the microscopes located at IBS and EMBL. The IGS laboratory possesses all necessary computing power (a total of 71 Nodes for 828 Cores and 150 Tb of storage including 3 SMP nodes with 256 Gb to 1 Tb of RAM and a massive GPU node with 18432 cuda cores over 4 NVIDIA RTX8000 )

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