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Reference : UMR7256-ESTGRO-006
Workplace : MARSEILLE 09
Date of publication : Friday, October 04, 2019
Type of Contract : FTC Scientist
Contract Period : 6 months
Expected date of employment : 1 December 2019
Proportion of work : Full time
Remuneration : Gross monthly salary between 2750 and 3840 € according to experience
Desired level of education : PhD
Experience required : Indifferent
The Genomics and Structural Information Laboratory (IGS) offers a VINCI (Franco-Italian University) post-doctoral position aimed at studying the details of the glycosylation machinery of the Mimiviridae of the 3 clades. This work is in continuity with the doctoral work between the Università degli studi di Napoli "Frederico II" and Aix-Marseille University, partly realized at the IGS and partly at the University of Naples (2016-2019) having also benefited from VINCI funding.
Mimivirus belongs to a family of giant viruses composed of three lineages, each capable of infecting Acanthamoeba. Their capsids measure about 500 nm and are surrounded by glycosylated fibrils, whose carbohydrate composition has been determined in the laboratories carrying this project. Mimivirus is the only virus for which the structure of polysaccharides decorating fibrils has been determined, and some of the enzymes responsible for their synthesis characterized (manuscript in preparation). Other members of the same family as Mimivirus have glycosylated fibrils composed of different monosaccharides, including rare sugars such as bacillosamine and rhamnosamine. The objective of this project is to determine the biosynthetic mechanism responsible for the synthesis of these sugars and the polysaccharides they compose, through the combined use of chemical, biochemical and bioinformatic methods.
-Doctorate in Biology and / or Chemistry
- Strong expertise in Biochemistry and Chemistry, knowledge in bioinformatics will be a plus.
The candidate must have defended his thesis in French-Italian cotutelle between December 10, 2016 and June 30, 2019.
The IGS laboratory is a pioneer and world leader in the study of giant viruses. We participated in the discovery of Mimivirus, the first giant virus and discovered the other three known families. The strength of the IGS laboratory lies in the coexistence of a team of bioinformaticians and a team of experimentalists, allowing to go from the isolation of new micro-organisms to their complete characterization by the combination of approach including genomics , transcriptomics and proteomics. The laboratory also has strong expertise in structural biology to determine 3D structures of viral proteins of interest by crystallography and combining crystallography and electron microscopy.
The laboratory is equipped for cell biology (1 L2, 6 PSM, 3 fluorescence microscopes including 1 Zeiss Axio); biochemistry (PCR, 1 PSM Microbiology, 3 incubators, 2 Aktä explorer 10S, 1 Aktä 3D, 1 Aktä Prime, Nanodrop, Qubit); structural biology (Mosquito crystallization robot, a diffractometer (Oxford diffraction Xcalibur PXUltra, SPD131 ThermoFischer speedvac) IGS has access to the 12 technical platforms of the Institute of Microbiology of the Mediterranean (IMM, AMU-CNRS FR3479, Personnel : 300) including an imaging platform fluorescence and electron microscopy (TEM, Cryo-TEM), NMR (600 MHz, cryoprobe), Proteomics, Protein expression and interaction study (1 Aktä prime, 1 Aktä Pure, 1 BLItz , ITC, Biacore) The iGS also has access to the imaging platform of Luminy campus (TEM and STEM) IBDM (Institute of Developmental Biology of Marseille) IGS belongs to BAG SUD and has access to synchrotrons (ESRF, Sun), electron microscopy platforms (Titan Krios cryo-electron microscope, K2 Summit live electron detector, Quantum LS imaging filter and Volta phase plate VPP). These microscopes on the line (CM01) (PSB) and at IBS and EMBL.
To apply candidates must submit a CV with the complete list of publications, a letter of motivation and at least one letter of reference. The candidate must have defended his thesis in French-Italian cotutelle between December 10, 2016 and June 30, 2019.
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