M/F Wide-field photothermal stimulated Raman microscopy

Job Description

Missions

Histology is commonly practiced in hospitals and involves taking a small tissue sample (biopsy), which is sliced, stained, and ultimately examined by a specialist to diagnose its cancerous nature. This process is time-consuming (1–24 hours) and hinders optimal patient management during surgery or endoscopic exploration.
Recently, we developed a wide-field coherent Raman microscopy technique using speckle illuminations and Coherent Anti-Stokes Raman Scattering (CARS) contrast to produce chemical images of biological samples [1]. However, this technique cannot generate histological images of biological tissues because the CARS contrast is affected by non-resonant background noise, which degrades the chemical images of CH₂ and CH₃ bonds required for constructing histological images [2].
This Postdoctoral research project aims to develop a new technology based on photo-thermal stimulated Raman scattering. This effect has been validated in a "point scanning" modality, where excitation beams are focused on the sample. Here, we propose to investigate the possibility of extending this photo-thermal stimulated Raman mechanism to wide-field imaging using speckle illuminations.
References :
[1] E. M. Fantuzzi, S. Heuke, S. Labouesse et al., "Wide-field coherent anti-Stokes Raman scattering microscopy using random illuminations," Nature Photonics (2023). https://doi.org/10.1038/s41566-023-01294-x

[2] B. Sarri, R. Appay, S. Heuke et al., "Observation of the compatibility of stimulated Raman histology with pathology workflow and genome sequencing," Translational Biophotonics 3, e202000020 (2021). https://doi.org/10.1002/tbio.202000020

Activity

The work program will focus on three objectives:
1. Simulate photo-thermal stimulated Raman contrast and assess its implementation in wide-field imaging with speckle illuminations.
2. Implement a proof-of-principle experiment demonstrating wide-field photo-thermal stimulated Raman imaging on test samples (liquids, polymer beads).
3. Explore the feasibility of wide-field photo-thermal stimulated Raman histology

Your Profil

Skills

Ability to lead a scientific project
Technical skills in optical instrumentation
Technical skills in computer interfacing with optoelectronic components
Data processing skills
Data presentation skills

Your Work Environment

This work will be in teh framework of the ERC SpeckleCARS

Compensation and benefits

Compensation

Between 3131,31 € and 4341,70 € gross depending on experience

Annual leave and RTT

44 jours

Remote Working practice and compensation

Pratique et indemnisation du TT

Transport

Prise en charge à 75% du coût et forfait mobilité durable jusqu’à 300€

About the offer

About the CNRS

The CNRS is a major player in fundamental research on a global scale. The CNRS is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to bring together different disciplines to address the most important challenges of the contemporary world, in connection with the actors of change.

CNRS

The research professions