Reference : UMR7241-MICZUG-003
Workplace : PARIS 05
Date of publication : Friday, November 18, 2022
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 9 December 2022
Proportion of work : Full time
Remuneration : 2661-3000€ monthly gross depending on experience.
Desired level of education : PhD
Experience required : Indifferent
We are seeking to recruit a post-doctoral fellow or an engineer (M/W) for one year (with one year possible extension).The scientific project focuses on the development of novel calcium imaging methods to record neuronal activity in freely-behaving mice, in order to investigate hippocampal dynamics underlying learning and memory. We have developed a fiberscope that allows for fast fluorescence confocal imaging (100 Hz) of GCaMP indicators during behavior and sleep. Building upon our prototype described in , our fiberscope can measure place fields and their evolution during multiple imaging sessions.
The candidate will extend the capacities of the fiberscope in two successive directions:
• Consolidate our recent developments leveraging the fast kinetics of GCaMP8, in order to reliably image fast hippocampal sequences during memory encoding and consolidation across successive learning days. Recordings will be compared between our fiberscope and a standard miniscope.
• Our optical system has the unique capability to perform targeted photoactivation simultanesouly with functional imaging. We will explore the ability to measure place coding using red calcium indicators (eg jRGECO1a) and combine it with targeted excitation or inhibition of specific cells using blue optogenetic actuators.
- Coupled implantation of the fiberscope and multisite electrodes
- Trainings and recordings
- Data analysis (coding, statistics)
- Collaborations with both teams and training labmates (e.g. PhD student, intern)
- Scientific writing and communication
- Neurosurgery in mice (AAV injections, implantation of chronic windows, implantation of extracellular electrodes)
- Recording neuronal activity using calcium imaging and electrophysiology (LFP)
- Animal training
- Data analysis
The projet takes place in the framework of a collaboration between the groups of Cathie Ventalon (IBENS) and Michaël Zugaro (CIRB, Collège de France, http://zugarolab.net). Our groups are located in the historic center of Paris and characterized by a diversity of backgrounds (physics, biology, engineering) and a friendly atmosphere.
Our teams combine a unique expertise in systems neuroscience and advanced optical imaging, internationally recognized and illustrated by numerous articles published in the most prestigious journals (Science, Neuron, Nature Neuroscience, etc.)
The offer is open and will remain open until the position is filled. Applicants should send their CV, a cover letter detailing their research experience and interests, and two letters of reference.
1 Dussaux et al. (2018), Scientific Reports 8:16262.
2 Oberto et al. (2021), Current Biology 32:1-13.e6.
3 Todorova and Zugaro (2019) Science 366:377-81
4 Drieu et al. (2018) Science 362:675-9
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