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Reference : UMR7232-DIDPEU-007
Workplace : BANYULS SUR MER
Date of publication : Wednesday, September 04, 2019
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 November 2019
Proportion of work : Full time
Remuneration : between 2 600 and 2 900 € based on the experience (gross monthly)
Desired level of education : 5-year university degree
Experience required : Indifferent
Vertebrate metamorphosis is a key stage in postembryonic development that allows the transition from larval to juvenile status, as is the case with frog tadpoles. Controlled by thyroid hormones, metamorphosis is characterized by the ecological, behavioral, morphological, and physiological changes necessary for the survival of juveniles in their new habitat. In coral fish, we lacked a model species to study this process as well as its molecular actors. For this, our team developed the clownfish Amphiprion ocellaris as a model species to determine morphological behavioral and molecular changes under control of thyroid hormones during metamorphosis. The analysis of the larval transcriptome has already allowed us to identify several genes whose expression is potentially regulated by thyroid hormones. As part of this project, we would like to test the effect of these hormones on the expression of these genes as well as the morphological and behavioral changes of clownfish larvae in the process of metamorphosis.
The candidate will have to analyze the gene list identified by trancriptomic analysis and identify those under control of thyroid hormones. For this, he will have to carry out pharmacological treatments to induce (by treatment with thyroid hormones) or to block (by treatment with goitrogens) the metamorphosis of the clownfish and to analyze the effect of these treatments on the expression of the genes. Concerning the blockage of the metamorphosis, the candidate will have to improve the protocol currently used in the team because no effect has been met for the moment on key genes of the signaling pathway of thyroid hormones.
In order to test the effect of thyroid hormones on the behavior of clownfish larvae, the candidate will have to set up behavioral tests. Recent work by the team has already shown that thyroid hormones control the visual perception of clownfish larvae, and we would like to determine if they control other behavioral responses such as predator perception, factor detection environmental like anemone etc ...
The candidate should have a good knowledge of developmental biology as well as molecular biology as well as phylogeny (RNA extraction, qPCR, mastery of molecular phylogeny software such as sea view, clustalX, Mr Bayes etc. .). He / she should also have knowledge of aquariology and larval rearing of marine fish as well as skills to develop behavioral tests in fish. Knowledge about the ecology of coral fish is a plus. The candidate will also be required to use the R software for all statistical analysis. The experimental level in animal experimentation is preferable.
Versatility, autonomy and independence are three essential qualities that the candidate will have to share as well as taking initiative and teamwork.
The candidate will join Vincent Laudet's team at the Oceanological Observatory of Banyuls-sur-Mer in the BIOM unit (UMR 7232 CNRS). The candidate will have a laboratory-based clownfish farm managed by the Research Aquarium Service, as well as an experimental room where he can perform pharmacological treatments and behavioral tests. Since this project involves working on living things, the candidate must be aware that he / she may be required to work on weekends, holidays and evenings depending on the experimental needs and requirements of the student. study model.
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