Missions

The engineer will contribute to enzyme engineering and enzyme evolution projects.

Activités

The candidate will participate in the establishment and standardization of the enzyme evolution pipeline, including advanced library generation and analysis tools, display and selection protocols, microfluidics, sequencing, automation and characterization.

Compétences

The candidate will have a background in molecular biology.
The directed evolution pipeline comprises many steps, including in vivo or in vitro protein expression, display and generation of smart mutant libraries, and next-generation sequencing. Candidates with one or more relevant skills, or with some experience in directed evolution are therefore particularly encouraged to apply.

General skills:
- Knowledge of directed evolution and expertise in molecular biology.
- A high level of scientific rigor.
- Self-organization, good communication skills, fluent English, production of scientific reports, data processing and management.
- Willingness to broaden skills and participate in research projects, scientific and technological watch.

Contexte de travail

The candidate will be integrated into a team that combines experiments based on molecular programming and quantitative modeling of high-throughput sequencing data.

The Gulliver laboratory is housed at ESPCI in the 5th arrondissement of Paris, and offers a friendly interdisciplinary environment for exploring original ideas and developing creative research projects.
This work builds on the group's extensive experience in high-throughput enzyme evolution, with a focus on in vitro protocols, as well as NGS sequencing, microfluidics, machine learning and molecular biology.

Contraintes et risques

Financing available over 1 year