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Portail > Offres > Offre UMR7053-YANBER-001 - H/F Postdoc Dévellopement de nanoparticules organiques photothéranostiques pour la thérapie anticancéreuse

H/F POSTDOCTORAL POSITION AT THE INTERFACE OF PHYSICAL CHEMISTRY AND PHOTOBIOLOGY FOR THE DEVELOPMENT OF ANTICANCER PHOTOTHERANOSTIC NANOPARTICLES

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : mardi 24 mai 2022

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General information

Reference : UMR7053-YANBER-001
Workplace : VANDOEUVRE LES NANCY
Date of publication : Tuesday, May 3, 2022
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 September 2022
Proportion of work : Full time
Remuneration : From 28 to 35 k€ per year
Desired level of education : PhD
Experience required : Indifferent

Missions

Over the last decade, photothermal therapy (PTT) has attracted increasing attention as a potential alternative to other classical therapeutic approaches. It involves molecules or nanoparticles absorbing photons upon NIR irradiation and generating heat through non-radiative relaxation pathways. To help the clinical translation of PTT, which is currently limited to a few early phase pilot trials, highly challenging research aspects concern the development of theranostic nanoparticles that provide efficient photothermal therapeutic effect in combination with a comprehensive image-guiding strategy by fluorescence/photoacoustic imaging (PAI). Indocyanine Green (ICG) is so far the only FDA-approved dye for fluorescent application and therefore stays on the frontline for fast pre-clinical and clinical PTT evaluation. Unfortunately, at the molecular scale it exhibits limited PTT efficiency, accumulation at the tumor site, and bio/photo-stability. Therefore, its formulation in supramolecular assemblies is of particular significance to improve its pharmacokinetics and PTT performances. In this context, our group is exploiting the physicochemical engineering of ICG into so-called J-type aggregates (i.e. Indocyanine green J-aggregates, IJA), which demonstrates better PTT efficiency and response as a contrast agent in PAI, as compared with ICG. However, because IJA quickly disassembles in complex biological media, research efforts are dedicated to the stabilization of IJA by appropriate formulation. In continuity with our ongoing research work, the project aims at 1) improve the comprehension of IJA chemical nature and structure at both molecular and self-assembled scales; 2) develop outperforming innovative IJA-based nanoparticles; 3) engineer these nanoparticles to incorporate a second fluorophore (e.g. commercial cyanines) for additional imaging outcomes, to targets in vivo intelligent fluorescent organic nanoparticles for dual image (PTT/PAI) -guided PTT strategy.

We offer a two-year postdoc position to work on the chemical to in vivo development of ICG-based phototheranostic nanoparticles as mentioned above. Within the frame of this project, both the physicochemical (nanoparticles formulation, chemical and photophysical analysis) and the biological (in cellulo and in vivo phototherapeutic activities evaluation) aspects will be investigated. Good expertise in photochemistry/photobiology will be required and valued in combination with high expertise in chemistry, physicochemical engineering, photophysics, and cellular/small animal biological studies of L2CM and CRAN. As a postdoctoral researcher, you will be attached to both laboratories, which are located in Vandoeuvre-les-Nancy, France.

Activities

Study of the structure and nature of IJA and its degradation products at molecular and self-assembled scale using chemical analysis technics (HPLC, NMR, Mass spectrometry).
• Preparation and characterization of fully organic IJA-based nanoparticles using physico-chemical engineering (e.g. surfactant-based nanoparticles, polymeric micelles, liposomes, nanoparticles constructed by electrostatic interactions).
• Assessment of photophysical properties of nanoparticles by photophysical technics (spectroscopy, fluorimetry, photothermal production setup)
• Investigation of photothermal activity, (photo)chemical stability in biological environments, evaluation of dark/photo toxicities, cellular uptakes/localization (FaDU cancer cell model and 3D cell models).
• In vivo investigation on mouse models bearing head and neck tumors (in vivo/ex vivo distribution, photothermal treatment, imaging using bimodal fluorescence/photoacoustic imaging equipment) – A formation for in vivo working with small animal models will be provided within the postdoc.
• Participation in the supervision of PhDs, engineers, and trainees - restoring the results, communication at international conferences, participation in writing of manuscripts.

Skills

You should hold a PhD in photobiology with prior experience in cell biology and at the interface with physical-chemistry/photophysics. Additional experience in formulation/nanoparticle synthesis and characterization, fluorophore synthesis and characterization (spectrofluorimetry, HPLC), or in vivo fluorescence/photoacoustic imaging would be appreciated. Creativity, autonomy and strong reliability are highly required, together with a strong interest in multidisciplinary approach. This project will give great opportunities to develop/extend competencies in photophysics, physical chemistry, cell biology, and associated characterization techniques with cutting-edge equipment. You are expected to be highly motivated and possess a great team spirit to take advantage to work in a leading research environment and potentially make breakthrough innovations in cancer treatments. All applicants must be able to communicate fluently in English.

Work Context

Information related to the host laboratories:
Laboratoire Lorrain de Chimie Moléculaire (L2CM, UMR 7053),http://www.l2cm.univ-lorraine.fr/l2cm/, Boulevard des Aiguillettes B.P. 70239 - 54506 Vandoeuvre les Nancy Cedex France. The L2CM is a mixed research unit (UMR7053) between CNRS and University of Lorraine, which integrates around 70 members geographically distributed between Nancy (Faculté des Sciences et Technologies, Campus Brabois Santé) and Metz (Institut de Chimie, Physique et Matériaux). The objectives of the laboratory are to explore and develop synthetic methods for innovative molecules and molecular materials for applications in various domains towards chemistry (drug design, catalysis), physics (energy, materials) and biology (drug delivery, imaging, therapy).
Centre de Recherche en Automatique de Nancy (CRAN, UMR 7039,http://www.cran.univ-lorraine.fr/, Campus Sciences BP 70239 54506, Vandœuvre-lès-Nancy. Created in 1980, CRAN is a joint research unit shared by the University of Lorraine and the CNRS (attach to INS2I and INSIS). It also hosts researchers from the Lorraine Cancer Institute (ICL) and the University Regional Hospital (CHRU). The laboratory conducts interdisciplinary research associating automation, signal and image processing with biology and medicine. For several years, CRAN/ICL researchers have been conducting multidisciplinary research aimed at increasing the effectiveness of therapeutic strategies that exploit the interactions of light with biological tissue.

You will be supervised by Yann Bernhard at L2CM and Henri-Pierre Lassalle at CRAN.

Constraints and risks

Standard constraints and risks of chemistry biology laboratories and high intensity laser manipulation

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