Missions

The person recruited will deploy molecular and cellular biology techniques within the ANR-DFG funded "DOLLI" project, a collaboration between a team from the Villefranche-sur-mer Developmental Biology Laboratory and colleagues from Göttingen (MPI and University). This project addresses oogenesis mechanisms in the jellyfish model species Clytia hemisphaerica.

Activités

- Produce and test molecular constructs allowing the expression of fluorescent proteins and mRNA in Clytia oocytes. This will involve standard molecular biology techniques such as PCR, cloning and mRNA synthesis.
- Participate in some microinjection and imaging experiments.
- Record results and Communicate experimental data with the project leaders and with collaborators.
- Participate in the maintenance of Clytia strains.
- Respect and ethical, health and safety rules.

Compétences

- Basic knowledge of animal biology, genetics, molecular and cellular biology and immunology
- Expertise in using standard molecular and cell biology techniques.
- Sequence manipulation software (e.g. BioEdit, Genious)
- Use of software to pilot and record data from specific pieces of apparatus.
- Organise your activities efficiently
- Legal and ethical frameworks for (basic concepts)
- Health and safety regulations
- English Language level B1

Contexte de travail

This 12-month contract is part of a collaborative ANR-DFG financed project between the "Cnidarian Developmental Mechanisms" team at the Villefranche-sur-mer Developmental Biology Laboratory and teams in Göttingen (at the MPI and the University). The project aims to develop imaging approaches to study the cell biology of oogenesis using the jellyfish Clytia hemisphaerica . In close collaboration with an AI and a researcher in the team, the person recruited will contribute to the activities of "Work Package 2: Developing a suite of specific molecular tools for probing the biology of oocytes".