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Reference : UMR6290-MARTRA-003
Workplace : RENNES
Date of publication : Wednesday, September 15, 2021
Type of Contract : FTC Scientist
Contract Period : 30 months
Expected date of employment : 1 January 2022
Proportion of work : Full time
Remuneration : between 2648 et 3054 € brut per month
Desired level of education : PhD
Experience required : 1 to 4 years
A post-doctoral position funded by ITMO cancer is available in the group of Dr. Marc Tramier at the Institute of Genetics and Development of Rennes (IGDR), France https://igdr.univ-rennes1.fr/en/marc-tramier-group. Research in this team relates to the use of state-of-the-art methods and techniques in quantitative fluorescence microscopy to investigate spatio-temporal regulation of protein-protein interactions and biochemical activities in live cells.
In this collaborative project with May Morris (IBMM Montpellier) and Frederic Bihel (LIT Strasbourg), the aim of our team is to develop a conformational FRET biosensor for CDK5 to screen allosteric inhibitors mode of action in living cells. To date, few allosteric inhibitors of protein kinases have been developed, although they are believed to be more selective than compounds targeting the active site. To address this issue, a fluorescent conformational protein biosensor was engineered (Morris team) and implemented to screen several libraries of small molecules (Bihel team) to identify allosteric modulators of CDK5, leading to identification of several hits of particularly attractive chemical and biological interest. In this project we will implement an orthogonal imaging assay to characterize these compounds based on a conformational FRET biosensor (Tramier team), enabling identification of allosteric inhibitors that target conformational activation of CDK5 in living cells to gain insight into their mechanism of action.
The successful candidate will be responsible for the generation of genetically encoded FRET biosensors (molecular biology), for the expression of these biosensors in the different context (cell biology) and the use of advanced FRET/FLIM in HCS microscopy to screen the different compounds (fluorescence microscopy).
We are seeking candidates who are ready to take on this innovative project in a very collegial and interactive environment. The desired candidate should have a strong background in molecular and cell biology to be able to conduct these aspects independently and a pronounced interest in fluorescence microscopy.
The Institute of Genetics and Development of Rennes is dynamic and expanding, with 15 teams representing 200 international researchers and staff, including several recently established young groups. Research at the IGDR covers a wide range of subjects in cell biology, taking advantage of most classical model systems while strongly promoting interdisciplinary approaches, and particularly interface between physics and biology. The institute also benefits from state-of-the-art core facilities, particularly in microscopy (Microscopy Rennes Imaging Center, MRic, http://microscopie.univ-rennes1.fr/index.html).
Rennes is the capital of Brittany, in northwestern France, with easy and direct access to Paris and Charles de Gaulle Airport (around 2 hours by train). Its rich tradition of cultural, musical and artistic events, as well as its close proximity to the coast, makes it a very welcoming and pleasant place to live.
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