Missions

The postdoctoral researcher will join the LUMOS project (Light-induced skin extracellular matrix remodeling through reactive oxygen species generation), funded by the French National Research Agency (ANR-23-CE18-0015). The project aims to develop calibrated photochemical approaches to modulate the human skin extracellular matrix.
The main mission will be to evaluate the effects of sublethal photodynamic therapy on skin tissue remodeling, particularly using reconstructed human skin models that mimic aging or wound-healing conditions.
This work takes place in a multidisciplinary framework combining chemistry, biophysics, and tissue engineering, with the goal of linking the photochemical properties of photosensitizers to the structural and functional responses of the skin tissue.

Activités

Culture human skin cells in 2D (monolayers) and 3D (reconstructed skin obtained by self-assembly approach).
Participate in the development and optimization of experimental protocols.
Implement calibrated photodynamic therapy protocols to induce tissue remodeling without causing cell death.
Characterize cellular responses using functional assays: migration, proliferation, barrier function, angiogenesis.
Analyze tissue and matrix modifications using histological, immunofluorescence, biochemical, and molecular approaches (qPCR, Western blot, ELISA, optical and electron microscopy).
Collaborate with project partners to evaluate the mechanical and structural properties of the tissue after treatment.
Contribute to the scientific dissemination of results (reports, publications, presentations) and provide occasional supervision of laboratory students.

Compétences

PhD in biology, cell and molecular biology, bioengineering, or a related discipline, ideally with experience in tissue engineering. Knowledge of skin biology and/or wound healing is a plus.
Experience in 2D and 3D cell culture.
Proficiency in molecular and biochemical techniques: qPCR, Western blot, ELISA.
Proficiency in cell biology and histology techniques: immunofluorescence, histology, optical and fluorescence microscopy.
Ability to analyze quantitative and qualitative data (statistics).
Strong interest in multidisciplinary research, with the ability to collaborate with chemists and physicists.
Experimental rigor, organizational skills, autonomy, and strong writing skills for preparing reports and scientific publications.
Ability to occasionally supervise students and work effectively in a collaborative environment.

Contexte de travail

The project is part of a Toulouse-based, tripartite, and multidisciplinary consortium including Softmat (UMR CNRS 5623), Toulouse University Hospital (CHU), and CIRIMAT.
The postdoctoral researcher will be based at Softmat, working in collaboration with an already recruited PhD student, supported by a biology technician, under the supervision of L. Gibot (CNRS Research Scientist).
The contract is expected to start between January and March 2026, depending on the candidate's availability.

Contraintes et risques

Not applicable