Missions

Working as part of the "Hematopoiesis and Immunotherapy" team (http://www.igmm.cnrs.fr/team/hematopoiese-et-immunotherapie/) and in close collaboration with the Clinical Hematology Department at Montpellier University Hospital, the engineer will be involved in the development of a project aimed at identifying and characterizing the factors impacting the efficacy of CAR-T lymphocytes used for the treatment of patients, particularly lymphoma patients, using ex vivo and in vivo approaches (primary human cells from patients or healthy donors and mouse models).

Activités

- Develop and conduct a panel of molecular and cellular biology techniques (primary cell culture, retroviral/lentiviral vector transductions, flow cytometry, Incucyte, PCR, microscopy, metabolic analysis, animal handling, etc.), and adjust experimental conditions to the scientific question
- Exploit and present analysis results in written reports and oral presentations
- Write and update technical protocols, guarantee their follow-up and quality, keep a rigorous laboratory notebook.
- Train new team members and contribute to technology transfer
- Maintain a scientific and technological follow up in our field of activity

Compétences

General, theoretical or disciplinary knowledge : - Immunology, Biochemistry (metabolism) - Cell biology - Language skills: oral and written comprehension level B1 to B2 for English. Operational skills : - Implement biology techniques, including a good knowledge of flow cytometry and cell culture. - Animal experimentation (animal handling, sampling and general knowledge of regulations). - Ability to organize daily work independently. - Report on activities. - Use IT tools for data analysis, formatting and recording. - Be motivated, dynamic, rigorous and conscientious in his/her work. - Work as part of a team and contribute to the life of the laboratory.

Contexte de travail

The Institut de Génétique Moléculaire de Montpellier (IGMM) is a joint research unit of the CNRS and the University of Montpellier, with a staff of 220 divided into 16 research groups. The IGMM is a multidisciplinary institute whose work has an international impact in both fundamental and applied molecular and cellular biology (www.igmm.cnrs.fr). The campus provides access to state-of-the-art scientific and technological facilities, particularly in genomics, imaging and flow cytometry.
The candidate will be assigned to the "Hematopoiesis and Immunotherapy" team codirected by V. Zimmermann and N. Taylor, but will also work closely with the Clinical Hematology Department directed by G. Cartron at Montpellier University Hospital.

Contraintes et risques

- Collaborative work carried out both in the IGMM research laboratory and in the clinical hematology department of Montpellier University Hospital.
- Work on human primary T cells (carried out in L2 laboratory, requiring PPE -overshoes, gowns, gloves- and the handling of human cells implies the need to take biological risk into account).
- Work on live animals (carried out in an animal facility, requiring PPE -overshoes, gowns, gloves, mask).
- Variability