Missions

The incoming post-doctoral researcher will be involved in a project on genomic imprinting, an essential epigenetic phenomenon in mammals. Particularly, the project investigates the regulation and role of asynchronous DNA replication at imprinted gene domains in mammals. He/she will use various approaches to assay replication timing, locus-specifically and genome-wide, in mouse embryonic stem cells and in differentiated cells. In addition, he/she will perform diverse molecular, biochemical and bioinformatics studies, and will apply functional genomics approaches to explore key regulatory sequences and trans-acting factors.

Activités

-Culture and differentiation of embryonic stem cells.
-Analysis of replication timing, DNA methylation and gene expression, data processing, bioinformatics. Fluorescence microscopy to visualize chromatids.
-Functional studies using diverse CRISPR-based approaches.

Compétences

The candidate needs to be familiar with DNA replication or chromatin research, and have theoretical and experimental experience in genetics and molecular biology, with knowledge of transcription, chromatin and/or functional genomics. Experience with bioinformatics would be welcomed as well.

Contexte de travail

The Institute of Molecular Genetics (IGMM in Montpellier is a 'mixed research unit' between the CNRS and the University of Montpellier, with about 180 employees working in 17 research groups, 9 common services and 9 state-of-the-art technological platforms (see http://biocampus.cnrs.fr ), with a highly international atmosphere. IGMM is a multi-disciplinary institute its research outputs having international fundamental and applied impacts in molecular and cellular biology. The 'Genomic imprinting and Development' laboratory, headed by Robert Feil, explores epigenetic mechanisms with a particular interest in genomic imprinting and lncRNAs, as well as in chromatin structuration in living cells. For further information, please see http://www.igmm.cnrs.fr/.

The host laboratory (R. Feil) seeks to recruit a highly motivated and dynamic researcher with several years of post-doctoral experience, with considerable experience in molecular biology and/or genomic studies, and preferably also with experience in bioinformatics.

Relevant publications of the host laboratory:
-- Dupont, C, Chahar, D, Trullo, A, Gostan, T, Surcis, C, Grimaud, C, Fisher, D, Feil R, Llères D. (2023). Evidence for low nanocompaction of heterochromatin in living embryonic stem cells. EMBO J. e110286.
--Noordermeer, D., Feil, R. (2020). Differential chromatin organization and gene activity in genomic imprinting. Curr. Opin. Genet. Dev., 61, 17-24.
-- Llères, D, Moindrot, B, Pathak, R., Piras, V., Matelot, M., Pignard, B., Marchand, A., Poncelet, M., Perrin, A., Tellier, V., Feil, R., Noordermeer, D. (2019). CTCF modulates allele-specific sub-TAD structuration and imprinted gene activity at the Dlk1-Dio3 and Igf2-H19 domains. Genome Biol., 20, 272.
-- Sanli, I., Lalevée, S., Camissa, M., Perrin, A., Rage, F., Riccio, A., Llères, D., Bertrand, E., Feil, R. (2018). Meg3 long non-coding RNA expression controls imprinting by preventing transcriptional upregulation in cis. Cell reports, 23, 337-348.272.
-- Prats-Puig, A., Carreras-Badoso, G., Bassols, J., Cavelier, P., Magret, A., Sabench, C., de Zegher, F., Ibanez, L., Feil, R, Lopez-Bermejo, A (2017). The placental imprinted DLK1-DIO3 domain: a new link to pre- and postnatal growth in humans. Am. J. Obstet. & Gynecology, 217, e1-350.
-- Kota, S.K., Lleres, D., Bouschet, T., Hirasawa, R., Marchand, A., Begon-Pescia, C., Sanli, I, Arnaud, P., Journot, L., Girardot, M. and Feil, R. (2014). ICR non-coding RNA expression controls imprinting and DNA replication at the Dlk1-Dio3 domain. Developmental Cell, 31, 19-33.