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Reference : UMR5525-ELSGEN-022
Workplace : LA TRONCHE
Date of publication : Wednesday, June 2, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 September 2021
Proportion of work : Full time
Remuneration : About 3768.64€ regarding experiences
Desired level of education : PhD
Experience required : 1 to 4 years
Investigation of the evolution of bacterial antibiotic resistance mediated by the genetic elements called Integrons and the bacterial lifestyle.
The activities of this project include: bacteriology, experimental evolution, molecular genetics, genomics analyses.The recruited post-doc will have to handle evolution experiments aiming at selecting E. coli strains with increased antibiotic resistance due to the evolution of ancestral integrons. He/she will have then to characterize the selected clones both at the phenotypic and genotypic level. The project will first consist in constructing molecular tools to mobilize artificial integrons in various genetic backgrounds that then will be used for evolution experiments. The selected constructs will then be propagated in different media with different antibiotic concentrations either in batch or in biofilm-mimicking conditions. The evolution of the populations will be investigated by recording MIC for specific antibiotics as well as integrons expression using reporter fusions. Selected clones will finally be fully characterize by whole genome re-sequencing in order to identify the mutations that have contributed to the emergence of high resistance level driven by integrons.
The recruited person will hold a Ph.D with a solid training and expertise in bacteriology, molecular bacterial genetics, experimental evolution, bacterial genomics analyses and genetics of antibiotic resistance. Ideally, he/she is expected to be used with bacterial cultures, molecular cloning, determination of antibiotic MIC, flow cytometry analyses as well as with bioinformatic tools allowing identification of mutations after genome from selected clones have been re-sequenced. A previous post-doctoral intership is welcome. An experience in the biology of bacterial biofilms would be a plus.
The TIMC laboratory brings together scientists and clinicians for the understanding and control of normal and pathological processes in biology and health. Its multidisciplinary activity contributes both to basic knowledge in these fields and to the development of systems to aid diagnosis and therapy. TIMC has about 300 members in 12 research teams, including about 160 permanent staff: researchers, teacher-researchers, engineers, technicians and administrative staff. The TrEE team has a strong expertise in experimental evolution and in the analysis of molecular mechanisms sustaining adaptation of bacterial populations. It therefore offers an optimal framework to develop this project, at all levels: scientific, human, and technical. The project consists in investigating the impact of sub-inhibitory concentrations of antibiotic and bacterial lifestyle (planktonic versus biofilms) on the evolution of resistance integrons. A partner with expertise in the biology of Integrons and bacterial biofilms, participates in this project funded by the French "Agence Nationale de la Recherche".
Constraints and risks
No specific risks. The researcher may have to spend some time in the partner laboratory in Limoges.
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