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Reference : UMR5306-RODANT-001
Workplace : VILLEURBANNE
Date of publication : Thursday, July 1, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 31 December 2021
Proportion of work : Full time
Remuneration : 2675 to 3084€ gross salary per month (for less than 2 years experience).
Desired level of education : PhD
Experience required : 1 to 4 years
A position of non-permanent researcher is open at the Institut Lumière Matière in Lyon, France (iLM - Université Claude Bernard Lyon 1, CNRS) in the framework of the European FET-OPEN project ARIADNE (see https://cordis.europa.eu/project/id/964553). The objective of the ARIADNE project is to develop a multi-analytical platform based on mass spectrometry instrumentation, and to achieve direct and instantaneous detection of intact viral particles exhaled by breath or present in water. ARIADNE incorporates state-of-the-art technological advances in breath aerosol sampling and post-ionization methods, as well as ultra-high mass non-destructive analysis of single bioparticles.
The fixed-term contract researcher hired at iLM-CNRS will be responsible for building on our local expertise in charge detection mass spectrometry (CDMS) of massive bioparticles. He/she will be responsible for the development and evaluation of the second generation CDMS system operating in stand-alone mode, analyzing viruses and bacteria using electrospray. Additional top-down analysis of single bioparticles will be performed in the CDMS system by multiphoton dissociation using a CO2 laser to produce high mass fragments.
The candidate will be in charge of setting up a new methodology to :
- Optmize the conditions of gas phase and ionization of bioparticles in native conditions.
- Design a calibration of the new charge detection mass spectrometer
- Design the analysis strategy for viruses and bacteria
- Implement photofragmentation experiments on bioparticles trapped with a CO2 laser
- Ensure data processing and scientific dissemination of results (writing articles in English, conferences)
The M/F candidate must have a PhD in physical chemistry or chemistry (bio/analytical). He/she must have experience in laser spectroscopy (action spectroscopy) and mass spectrometry, in particular mass spectrometry of protein complexes: expertise in native mass spectrometry will be particularly appreciated. Experience in MS instrumentation is an important asset. The candidate must be comfortable with handling biological samples (large proteins and assemblies): purification, storage and preparation for native MS and have a critical eye for assessing the structural integrity of systems.
he ILM is a joint research unit between the University of Lyon (UCBL) and the Centre National de la Recherche Scientifique (CNRS). It comprises about 300 people, including researchers, teacher-researchers, research support staff, doctoral students and post-doctoral students. The ILM is composed of 17 research teams organized along six thematic axes, addressing various topics ranging from solid state physics and nanoscience to ultrafast optics and matter-light interaction. The iLM is located on the Lyon Tech La Doua campus in Villeurbanne.
The research activity will be carried out under the supervision of Rodolphe Antoine of the Spectrobio research group. This 24-month position funded by Europe will be located at the Institut Lumière Matière (iLM site ISA).
Constraints and risks
Laser and aerosol security.
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