Missions

Synthesis of an mRNA encoding a protein of interest and formulation into a lipid nanovector.
Characterization of the biological activities of the complex in in vitro models using transcriptomic, proteomic and fluorescence microcopy imaging approaches.
ANR THERALVEO project (https://anr.fr/Projet-ANR-22-CE18-0040)

Activités

- In vitro transcription and mRNA purification
- Formulation of liponanoparticles (LNPs) using a microfluidic device
- Physico-chemical characterization of mRNA/LNP complexes by DLS (Malvern Zetasizer Ultra)
- Cell culture of human and murine lung cell lines
- Quantitative PCR
- Western blot
- Omics analysis of microRNA
- Confocal microscopy
- Data analysis and writing of publications in English

Compétences

- expertise in mRNAs (structure, stability, in vitro synthesis)
- Mastering of omics analysis tools (R or other software)
- Know-how in 2D cell culture, knowledge of lung cells would be appreciated
- Skills in nanovector formulation would be appreciated
- Good command of English (reading, writing and speaking) (interaction with a group in Germany)
- Trainings with microfluidic device and confocal microscopy will be provided.

Contexte de travail

The position is based in Lyon 8ème in the Faculty of Medicine-Pharmacy (Rockefeller building). The on-site research group is made up of 7 permanent staff and 6 to 10 non-permanent staff belonging to the Tissue Biology and Therapeutic Engineering Laboratory. The person recruited will interact with the rest of the team located at Gerland Lyon 7ème (20 minutes by metro), and with the partner team of the ANR project (IMRB in Créteil, and the Institute for Lung health, Giessen, Germany).

Le poste se situe dans un secteur relevant de la protection du potentiel scientifique et technique (PPST), et nécessite donc, conformément à la réglementation, que votre arrivée soit autorisée par l'autorité compétente du MESR.

Contraintes et risques

The position involves handling biological products of human and murine origin.