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Portail > Offres > Offre UMR5305-CATMOA-007 - Chercheur(e) post-doctorant(e) H/F en biochimie et biologie structurale de la biosynthèse des collagènes

Post-doc position (M/F) Biochemistry and structural biology of collagen biosynthesis

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : mardi 13 décembre 2022

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General information

Reference : UMR5305-CATMOA-007
Workplace : LYON 07
Date of publication : Tuesday, November 22, 2022
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 January 2023
Proportion of work : Full time
Remuneration : Gross salary between 2833 and 4003 euros/month depending on experience
Desired level of education : PhD
Experience required : 1 to 4 years

Missions

The post-doctoral fellow will perform and analyze biochemistry and structural biology experiments in relation with the study of the proteolytic maturation complex(es) of fibrillar collagens.

Activities

- Perform cloning and mutagenesis experiments
- Produce recombinant proteins from mammalian cells
- Purify proteins on FPLC systems and perform quality control
- Optimize the production of various protein complexes for cryo-electron microscopy (cryo-EM)
- Participate in cryo-EM data collection and analysis (in collaboration)
- Perform protein/protein interaction studies by SPR or thermophoresis
- Perform protein assays by western-blot (classical or simple western), ELISA and targeted mass spectrometry (PRM)
- Report results (publications, project meetings, group meetings, conferences etc.)

Skills

- Excellent theoritical and practical knowledge of molecular biology and protein biochemistry
- Experience in running protein-protein interaction analyses (ideally SPR) and biochemical assays to measure protein concentrations (ELISA, mass spectrometry)
- Knowledge in structural biology; previous experience in cryo-EM would be an advantage
- Knowledge in extracellular matrix biology
- Fluency in English
- Experience in scientific writing

Work Context

The biosynthesis of fibrillar collagens is severely deregulated in fibrosis, the common and deleterious outcome of a great diversity of tissue injuries. A better understanding of the various steps leading from the synthesis of individual procollagen chains to collagen fibrils would greatly benefit the development of new therapeutic tools. In the P3-complex project, we focus on the C-terminal proteolytic maturation of fibrillar procollagens in order to :
- better understand, at the molecular level, how the various components of the maturation complex (substrate, protease, regulatory proteins) associate and work together. This is done mainly by cryo-electron microscopy and through a combination of biochemical/biophysical approaches.
- learn more about how this complex affects tissue biology and extracellular matrix remodelling using transcriptomics, targeted mass spectrometry and biochemical assays.
This project is funded by the French Research Agency (ANR) and is performed in collaboration with the OPIC (Oxford Particle Imaging Center) facility in Oxford for cryo-EM and with the University Hospital of Dijon for fibrosis samples. It will contribute to optimize innovative tools, currently under development in the group, to diagnose and treat fibrosis.
Most of the work will be performed at LBTI (Tissue Biology and Therapeutic Engineering Laboratory, UMR5305 CNRS / University of Lyon). LBTI is a multi-disciplinary laboratory focused on tissue biology (homeostasis, stress responses, aging etc) and on the indentification of therapeutic targets.

Constraints and risks

Work in BSL2 lab

Additional Information

CV must include the publication list of the candidate and the names of at least two references.

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