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Portail > Offres > Offre UMR5305-CATMOA-006 - Chercheur(e) post-doctorant(e) H/F en biochimie et biologie structurale de la biosynthèse des collagènes

Post-doctoral fellow (M/F) in biochemistry and structural biology of collagen biosynthesis

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : mardi 23 août 2022

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General information

Reference : UMR5305-CATMOA-006
Workplace : LYON 07
Date of publication : Tuesday, August 2, 2022
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 October 2022
Proportion of work : Full time
Remuneration : Between 3821 and 3993 € gross monthly salary depending on experience
Desired level of education : PhD
Experience required : 5 to 10 years

Missions

The selected candidate will perform biochemistry and structural biology experiments in relation with the proteolytic complexes involved in collagen maturation and fibrillogenesis. He/she will work with different types of samples including purified proteins, cell cultures, plasma and tissue biopsies.

Activities

- Perform cloning and site-directed mutagenesis experiments
- Express recombinant proteins in mammalian cell lines
- Purify proteins using FPLC systems and perform their quality control
- Optimize the production of various protein complexes for cryo-electron microscopy (cryo-EM)
- Participate in cryo-EM data collection and analysis (in collaboration)
- Carry out protein-protein interaction studies by SPR or thermophoresis
- Perform biochemical assays to measure/compare protein amounts by Western blot, ELISA or trageted proteomics (PRM)
- Check published litterature in the field
- Make oral and written reports (project meetings, group meetings, conferences etc)
- Participate to common lab tasks (cell culture room cleaning, consumable stock monitoring etc)

Skills

- Excellent theoritical and practical knowledge of molecular biology and protein biochemistry
- Experience in running protein-protein interaction analyses (ideally SPR) and biochemical assays to measure protein concentrations (ELISA, targeted mass spectrometry)
- Knowledge in structural biology; previous experience in cryo-EM would be an advantage
- Knowledge in extracellular matrix bology
- Fluency in English (collaboration with a group in Oxford)
- Experience in writing scientific articles

Work Context

The work will mainly take place at UMR5305 (LBTI) in Lyon Gerland but short stays at the OPIC (Oxford Particle Imaging Center) facility of the STRUBI lab in Oxford are planned. LBTI is a pluridisciplinary laboratory with interest in tissue biology (homeostasis, stress response, aging etc) and therapeutic target identification. The host team works on extracellular metalloproteases and extracellular matrix in the context of tissue repair and fibrosis. The project will include biochemistry, structural biology but also analysis of human and murine samples. The selected candidate will have access to the cutting-edge facilities of the SFR Biosciences and the SFR Santé. The project is funded by the French Research Agency (ANR).

Constraints and risks

Cell culture work in BSL2 environment. Rare possible work on week-end and holidays for cell culture maintenance.

Additional Information

The post-doctoral position can be a stepping stone to become a permanent CNRS researcher (competitive exam)

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