Intitulé de l'offre : Neuroscience researcher "Nanometric-scale study of the organisation of individual proteins in cellular adhesive structures" M/W (H/F)
Référence : UMR5297-MELDES0-189
Nombre de Postes : 1
Lieu de travail : BORDEAUX
Date de publication : mardi 14 novembre 2023
Type de contrat : CDD Scientifique
Durée du contrat : 12 mois
Date d'embauche prévue : 1 janvier 2024
Quotité de travail : Temps complet
Rémunération : Between €2905 gross and €3331 gross depending on experience
Niveau d'études souhaité : Niveau 8 - (Doctorat)
Expérience souhaitée : Indifférent
Section(s) CN : Molecular and cellular neurobiology, neurophysiology
We are looking for a candidate with skills in super-resolution imaging and tracking of individual proteins on primary cells (macrophages, neurons) in culture and in vitro preparations of cell membranes. The aim of this project is to study the organisation of individual proteins in cellular adhesive structures at the nanometric scale.
Conducting documented experiments in cell biology techniques (isolation and primary culture: murine neuronal cells, human and murine macrophages), preparation of cell membranes in vitro from living cells, molecular biology, biochemistry and protein engineering (development of genetically encoded probes) to understand the organisation of mechano-sensitive cell structures on nano-controlled substrates (nanoimprinting, nano-fabrication). Image acquisition using super-resolving microscopy and computer-based image analysis.
We are looking for a candidate with a deep interest in neuroscience, cell mechanobiology and their understanding at the cellular and molecular level.
The candidate should have solid experience in imaging primary cells (neuronal or macrophages) and in vitro biochemical approaches. They should also be familiar with nano-fabrication techniques, surface chemistry and super-resolution cell imaging. The use of scientific computing tools for image and data analysis is a prerequisite. The candidate must hold a PhD in neuroscience or cell biology and have good oral and written communication skills in English.
Contexte de travail
Created on 1 January 2011, the Institut interdisciplinaire de neurosciences (IINS) is a joint research unit attached to the CNRS and the University of Bordeaux. With 170 staff in 14 teams, IINS combines multidisciplinary skills to study the molecular and cellular mechanisms involved in brain activity. The Institute develops innovative methods in imaging, chemistry, physiology and computer science. As part of Bordeaux Neurocampus, IINS is one of the major players in the neuroscience community in Aquitaine.
Contraintes et risques
Possibility of working shifts