Faites connaître cette offre !
Reference : UMR5297-MELDES0-019
Workplace : BORDEAUX
Date of publication : Wednesday, October 02, 2019
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 November 2019
Proportion of work : Full time
Remuneration : Between 2617.05 et 3017.31€ monthly gross according to experience
Desired level of education : PhD
Experience required : 1 to 4 years
The goal of this project is to develop new rapid super-resolution microscopy modalities (RESOLFT, STED) to study cell adhesion in cellular and multicellular contexts. By combining these innovative techniques our goal will be to understand the mechanisms responsible for the formation, stabilization, and disassembly of adhesive structures. We will reveal the mechanisms responsible for mechano-transduction within these structures and study the molecular mechanisms of activation of integrins in tumor cells.
The candidate will have to use a wide range of experimental approaches at the interface between Cell Biology, Biochemistry and Physico-Chemistry:
1 / Photonic imaging of super-resolution RESOLFT, STED, PALM) and tracking of individual proteins.
2 / Surface chemistry and micro-fabrication techniques for micro-patterning innovative stretchable substrates, compatible with super-resolution imaging and tracking of individual proteins, with which we can mimic the different physical parameters of the extracellular matrix.
3 / Cell line cultures in which we will control the expression of specific integrins and their associated regulators by the genomic editing technique by the CRISPR / Cas9 strategy. We will notably express the wild proteins but also mutated versions.
The candidate will have to set up the corresponding methods of analysis and also conduct bibliographic analyzes, writing articles and projects, as well as presenting his results to an international audience
Cellular biology, biochemistry, physico-chemistryn photonic imaging super resolution
Established in January 2011, the Interdisciplinary Institute for Neuroscience (IINS) parent institutions are the CNRS (French National Center for Scientific Research) and the University of Bordeaux.
With 160 people and 12 research teams, IINS deploys multidisciplinary expertise to study molecular and cellular mechanisms involved in brain activity. The Institute develops innovative methods in imaging, chemistry, physiology and computer science. IINS is a major player in Aquitaine's neuroscience community and a leading component of Bordeaux Neurocampus and BRAIN, a cluster of excellence in Neuroscience.
Constraints and risks
Possibility to work in moved schedule
We talk about it on Twitter!