Missions

The post-doctoral fellow will join the TIGER (Translational and Integrative Group in Epilepsy research) team at the Lyon Neuroscience research center, as part of the ANR PRIMASENSE project to develop electrochemical microsensors for monitoring cerebral metabolism in non-human primates. The post-doctoral fellow will be tasked with developing glucose and lactate biosensors to be implanted in vivo in the brains of rodents, macaques or baboons housed in a Paris laboratory.

Activités

- Perform intracerebral electrochemical recordings to monitor changes in glucose, lactate and oxygen concentration over time using microbiosensors. Perform surgery on anesthetized rodents for preclinical testing.
- Manufacture microelectrodes and functionalize them to obtain enzymatic biosensors;
- Perform surface functionalization by evaporation or spraying - Perform electrochemical characterization of enzymatic microelectrodes by voltametry, amperometry.
- Characterize surfaces by scanning electron microscopy or Raman spectroscopy.

Compétences

-Knowledge of biology, neuroscience and laboratory techniques. Knowledge of electrochemical or electrophysiological recording techniques in rats;
-Knowledge of regulations relating to activities on animals, and up-to-date training/authorizations, in particular Training in Animal Experimentation Project Designer Level (Level I) and training in experimental surgery.
-Desired field of training:
o Neuroscience
o Animal experimentation (decree 2013-118 of February 1, 2013)
o Small animal surgery desirable.

Contexte de travail

This ANR-funded research project is the fruit of collaboration between the TIGER (Translational and Integrative Group in Epilepsy research) team at the Lyon Neuroscience Research Center (CRNL), the Brain and Spinal Cord Institute, the AMPERE laboratory in Lyon, and the Translational Research and Innovation in Medicine and Complexity (TIMC) laboratory in Grenoble. The post-doctoral fellow will be based at the CRNL, but will be required to visit both the Paris and Grenoble sites of the collaborating teams. The four laboratories CRNL, AMPERE, ICM and TIMC are centers of excellence in the fields of neuroscience and metabolism (CRNL, ICM) and electrochemistry and surface chemistry (AMPERE, TIMC). They are jointly developing innovative approaches to monitoring cerebral metabolism in animals, with a view to future applications in the human clinic. They have access to top-level research infrastructures in terms of animal research and surface and sensor engineering.

Contraintes et risques

Chemical risk
Biological risk
Occasional care of animals, possibly at weekends