Faites connaître cette offre !
Reference : UMR5286-NICAZN-001
Workplace : LYON 08
Date of publication : Monday, September 14, 2020
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 November 2020
Proportion of work : Full time
Remuneration : between 2675,28 € and 3084,60 €
Desired level of education : PhD
Experience required : 1 to 4 years
The candidate will co-develop an innovative system dedicated in mimicking in vivo Stem Cell (SC) “niche”. He or she will identify optimal SC culture conditions in order to generate high quality 3D organoids and tumoroids paving the way for future ex vivo augmented physiopathology modelling. Impact of cellular microenvironment on the stemness signature and downstream pathways by molecular biology and biochemistry will be investigated. This work will be performed on ex vivo models from lung and intestinal mouse tissues and cancer patients' samples (normal and tumoral). Finally, the candidate will be involved in writing reports, publications and grant applications. Traveling abroad and meeting with industrial partners will be also included.
- 3D cell culture
- cell viability assays
- western blotting and qPCR
- Academic background (evidenced by training and publications) is required with notably an extensive experience in cancer and stem cell biology, especially molecular and cellular assays and cell viability assessment. Moreover, the candidate should have a robust experience in 3D organoid cell culture.
- The candidate should be highly self-motivated, with strong communication and interpersonal skills and the ability to work independently. Experience in entrepreneurship and scientific valorisation will be considered as a plus.
Today, stem cells (SC) and their direct derivatives offer promising perspectives in regenerative medicine as well as in novel therapeutic cancer strategies. Actually studying this specific cell type will help in understanding disease development, cancer drug resistance and hopefully be as source of healthy cells in order to replace their impaired counterparts within a given patient. Because of tremendous amount of publicly available protocols, isolating SCs doesn't constitute a critical step anymore. Nonetheless, due to their sensitivity to microenvironmental changes, cultivating SCs in 3D organoids still remains challenging and researchers face technical issues in maintaining stemness phenotype to generate robust data. To overcome these obstacles, standard SC culture conditions need to be further improved to maintain their culture in long term concomitant with durable genetic and phenotypic stabilities.
This project will be developed in the Dr. T Renno's team, affiliated to the Cancer Research Centre of Lyon (CRCL – INSERM U1052 / CNRS 5286).
Constraints and risks
- Ex vivo approaches with human samples (normal and tumoral tissue).
- Confidentiality of the project and result is mandatory.
We talk about it on Twitter!