Reference : UMR5249-OLISEN-002
Workplace : GRENOBLE
Date of publication : Wednesday, September 21, 2022
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 February 2023
Proportion of work : Full time
Remuneration : Gross salary will be between 2805 and 3224 € / month, function of experience
Desired level of education : PhD
Experience required : 1 to 4 years
Lanthanide complexes with push-pull antennas for the sensitization of their luminescence have demonstrated their high potential for cell imaging, especially due to their biphotonic absorption properties, which allows excitation in the near infrared. In the frame of the ONCOVIEW project, we aim at developing luminescent lanthanide bioprobes for biological imaging of cells or small animals that operate in the near infrared for both excitation and emission. In this respect, we will design peptide-lanthanide complex conjugates in which the peptide serves as targeting unit to vectorize the lanthanide complex into cells. The recruited postdoc will have two objectives: (1) the synthesis and characterization of europium and ytterbium-based probes and their assessment for biphotonic microscopy of live cells and (2) the modification of these probes to sense hypoxia which characterizes interior of solid tumors.
The recruited postdoc will be in charge of the synthesis of some macrocyclic ligands with push-pull antennas for lanthanide binding, of the synthesis of conjugates, of spectroscopic studies, especially luminescence, for the characterization of the probes in aqueous solution and of cell microscopy.
We are looking for a bioinorganic Ph.D chemist with a strong experience in organic synthesis, in coordination chemistry in water and in spectroscopy, including optical ones. The selected candidate must master purification of organic compounds and/or biomolecules by chromatography techniques. Knowledge in fluorescence, NMR and mass spectrometry will be appreciated.
The recruited postdoc will join the “Metallopeptides” group of the Chemsitry and Biology of Metals lab. (joint lab from CEA, Univ. Grenoble Alpes and CNRS) led by Olivier Sénèque. The project is within the frame of the ONCOVIEW project. Other partners of this project are Raphaël Tripier (Univ. Bretagne Occidentale, Brest, France), d'Olivier Maury (ENS, Lyon, France) et de Magali Gary Bobo (IBMM, Montpellier, France).
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