Reference : UMR5248-PIEMAI-001
Workplace : PESSAC
Date of publication : Tuesday, April 26, 2022
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 October 2022
Proportion of work : Full time
Remuneration : €2 663,79 / €3 069.06
Desired level of education : PhD
Experience required : 1 to 4 years
The candidate will join the newly established team of Dr. Maisonneuve at the European Institute of Chemistry and Biology (IECB) to investigate the structural basis of the regulation of protein kinases and pseudokinases receptors in human signaling by using cryo-EM.
- Molecular biology (design and conception of plasmids for protein expression).
- Protein expression in eukaryotic cells (insect and human cells)
- Purification of membrane proteins
- Structural characterization of membrane proteins by cryo-EM.
- Functional and biophysical characterization of proteins in vitro and in cells (enzymology, ITC, Fluorescence polarization)
- Expertise in protein biochemistry (ideally in membrane proteins)
- Single-particle electron microscopy (cryo-EM)
- Protein expression in eukaryotic cells (insect and human)
- Ability to work independently and as a team member.
- Use of detergents, lipids, nanodiscs and amphipols.
The candidate will join an exciting and multidisciplinary scientific environment with on-site access to cutting-edge facilities in cryo-EM (housing a 200kV Arctica equipped with a K2 summit camera and soon to come 200kV Cryo-electron microscope equipped with an energy filter and last generation direct detector), X-Ray crystallography, NMR, Biochemistry, Biophysics, molecular biology, and cell biology. The project involved international collaborations with biologists for complementary characterization of protein function in cells and in mice.
Constraints and risks
The position is funded for 2 years, but the candidate is encouraged to apply for additional postdoctoral funding.
Interested applicants should submit a cover letter, CV and contact information of 2 references
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