General information
Offer title : Post-doctoral position in Molecular Mechanisms of Bacterial Secretion Systems M/F (H/F)
Reference : UMR5248-PETKRA-012
Number of position : 1
Workplace :
Date of publication : 27 January 2026
Type of Contract : Researcher in FTC
Contract Period : 14 months
Expected date of employment : 8 March 2026
Proportion of work : Full Time
Remuneration : between 3401€ and 4216€ gross per month according to enxperience
Desired level of education : Doctorate
Experience required : 1 to 4 years
Section(s) CN : 22 - Molecular and structural biology, biochemistry
Missions
A post-doctoral researcher position in the molecular mechanisms of bacterial secretion systems under control of the second messenger c-di-GMP. Bacterial secretion systems are membrane macrocomplexes that play an important role in biofilm formation, bacterial cell physiology and pathogenicity, and are important targets in the development of new anti-infective drugs. This project aims to understand the molecular mechanisms of the function and the pharmacology of these macromolecular systems.
This position is assigned to the CBMN laboratory (UMR5248), within the team of Dr. Petya Krasteva whose theme is "Structural Biology of Biofilms"
Activities
- Development of protocols for the expression and purification of proteins and membrane, cytosolic and / or periplasmic complexes in different heterologous systems. Including small and large scale cultuures.
- Development and realization of solubilization and protein stability tests, as well as functional assays.
- Perform protein crystallization screens and contribute to the design of crystal optimization methods.
- Data collection at synchrotron facilities in France and abroad (X-ray diffraction and state-of-the-art cryo-electron microscopy (cryoEM)
- Preparation of samples for cryo-EM, in-house data collection and analysis
- Analysis of the scientific literature, preparation of presentations, scientific communication
Skills
Recommended skills
- Production and purification of plasmid DNA in milligram quantities.
- Cloning, mutagenesis, design of expression constructs and molecular biology protocols
- (Membrane) protein purification, including high-throughput solubilization screens and liquid chromatography techniques.
- Protein biochemical characterization including knowledge in enzymology.
- Protein crystallization, including experience with high-throughput crystallization screening robots, and crystal optimization techniques.
- X-ray diffraction data collection and structure determination.
- Experience with single-particle electron microscopy, including cryo-EM.
- Experience with cryo-EM data processing.
- Experience in training students and postdocs -Experience in organizing travels to synchrotrons or other facilities for data collection, as well as, handling diffraction/cryo-EM data.
Training in the above will be provided. Experience is preferable but not obligatory.
Work Context
In October 2019, Dr. Krasteva's team joined the European Institute of Chemistry and Biology in Pessac. It is an incubator for project teams working on chemistry and biology. This institute brings together 13 research teams, representing approximately 200 staff. The post-doctoral researcher will benefit from an extremely stimulating scientific environment and access to technological platforms.
The offer is for a 14-20-month contract funded on an ANR project with possibility to extend depending on the recruitee's performance and experience.
Constraints and risks
Standard risks for biological research labs (Biosafety levels 1&2). Safety protocols established and to be observed.
Additional Information
This is an equal opportunity employment. Researchers from under-represented groups are encouraged to apply.