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Reference : UMR5244-DIAMER-002
Workplace : MONTPELLIER
Date of publication : Monday, July 19, 2021
Type of Contract : FTC Scientist
Contract Period : 18 months
Expected date of employment : 1 November 2021
Proportion of work : Full time
Remuneration : between 2700 and 3100 € gross/month
Desired level of education : PhD
Experience required : 1 to 4 years
Host/pathogen interactions are characterized by a co-evolutionary dynamics through which the two partners impose strong selective pressures on each other. Among the mechanisms involved, recent studies have shown that genetic and epigenetic inheritance should be taken into account as a driver of phenotypic variation and therefore of host / pathogen coevolution. Crassostrea gigas, the most exploited oyster species in the world, has been facing the Pacific Oyster Mortality Syndrome (POMS) since 2008. This disease, which has become panzootic, represents a threat to oyster farming worldwide but display all the characteristics of a good model for studying the mechanisms of rapid adaptation to pathogen emergence. Indeed, recent data have shown that the resistance of oysters to OsHV-1 µvar virus rely on several inherited but non-exclusive molecular mechanisms, involving genetic and non-genetic (epigenetics and microbiota) bases. The objectives of this project will be to study the molecular bases of oyster resistance to POMS through the triptych genome, epigenome and transcriptome. The responses to this objective will provide resistance markers that would be used in marker-assisted selection programs and, new empirical data to enrich the knowledge on the role of genetic and non-genetic inheritance in species adaptability to pathogen emergence
To study the molecular basis of oyster resistance to POMS we will study the triptych genome, epigenome and transcriptome. This will require carrying out: i) experiments in controlled environments , ii) molecular biology (DNA / RNA extraction, sequencing library construction, etc.), iii) bioinformatic processing of sequence data (filtering, mapping, SNP calling, Methylation calling…), iv) implementation of biostatistical approaches for the identification of the association between CpG / SNP / DEGs / molecular phenotype/ Resistance to the disease, v) result presentation in team meetings and congresses, vi) writing of scientific reports and articles.
The candidate will hold a PhD in genomics, (epi)genetics, molecular ecology or bioinformatics. He / she will have good knowledge in (epi)genomics, quantitative genetics and bioinformatics. Experience in phenotype / (epi)genotype association studies (EWAS, GWAS, QTL, eQTL, methQTL…) will be appreciated. The candidate will be involved in a multidisciplinary consortium; he / she should be curious about other disciplines and have good oral and written communication skills.
The candidate will join the Microevolution of Interactions in the Antropocene team of the Interactions Hosts Pathogens Environments laboratory at the Montpellier area. He / she will have to interact regularly with the participants of the GestInnov project within the laboratory but also with the external partners.
Constraints and risks
Field trips and travel for meetings will have to be carried out. The candidate will have to handle potentially hazardous chemicals and biological agents but these won't represent a danger if good laboratory practices are respected.
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