General information
Reference : UMR5239-VINMOC-001
Workplace : LYON 07
Date of publication : Friday, June 17, 2022
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 August 2022
Proportion of work : Full time
Remuneration : 3000-3500€ brut/month
Desired level of education : PhD
Experience required : 1 to 4 years
Missions
The mission is part of a research project of the team on the dynamics of chromatin linked with DNA repair by base excision (BER) funded by ANR.
The objective of the person recruited as a post-doc for one year is to study the dynamics of DNA repair proteins in the nucleosome in the framework of the ANR funded project DYPROSOME (https://anr.fr/Projet-ANR-21-CE45-0032).
Activities
- Carry out molecular biology experiments (cloning, PCR, plasmid management and manipulation, sequence design, preparation of DNA substrates of size 200-2500 bp containing unique base modifications at specific sites); biochemistry (overexpression and purification by FPLC of recombinant WT and mutated proteins in bacteria such as corpus histones and linkers, DNA glycosylases etc.)
- In vitro reconstitution of mono- and oligo-nucleosome substrates by the salt gradient dialysis technique. Preparation of nucleosome-glycosylase complexes for structural studies with cryo-EM.
-Perform in vitro repair studies with purified proteins (glycosylases) and cell extracts in mono- and oligo-nucleosomes
-Collaborate with other members of the consortium in the framework of the project, but also with team and laboratory members working on other topics of common interest.
- Exploit and present the results of the analyses, ensuring their quality and reproducibility.
- Write experimental reports and protocols.
- Ensure the application of the principles and rules of hygiene and safety.
- Ensure a scientific and technological watch in its field of activity.
- Participate in the dissemination of results in the form of oral presentations and publications.
- Supervision of trainees at Bachelor and Master levels; training of personnel in the techniques used in the laboratory and in the functioning of the team.
Skills
Knowledge:
- PhD level in molecular biology and chromatin biochemistry. Some experience as a post-doc in the field is highly desirable.
- Extensive knowledge in epigenetics and chromatin, DNA repair mechanisms in the context of chromatin, in particular through the BER pathway. Good knowledge and some experience in physical chemistry, interaction biology and structural biology will be a plus.
Know-how:
- Excellent command of molecular biology and chromatin biochemistry techniques. In particular, prepare nucleosome samples for biochemical, physicochemical (SAXS, analytical centrifugation) and structural (cryo-EM) analyses.
-Experience in the field of DNA repair in a chromatin context.
- Optimization of experimental protocols.
- Organization, data analysis and good mastery of data processing software.
- Reporting and presentation of results in team meetings.
- Very good level in English.
Personal skills:
- Experimental rigor.
- Excellent organizational skills.
- Autonomy in the planning and management of experiments.
- Analytical and scientific reasoning skills.
- Relational skills and ability to work in a team.
Work Context
The Jalinot/Mocquet team at the Laboratory of Biology and Modeling of the Cell LBMC, ENS Lyon. The person recruited will be supervised by Dimitar Angelov, DRE CNRS.
Constraints and risks
No particular constraint or risk, however the ability to work with 32P radioactivity in a radioactive room is required on an episodic basis.
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