Reference : UMR5239-ISASER-015
Workplace : LYON 07
Date of publication : Wednesday, June 22, 2022
Type of Contract : FTC Technical / Administrative
Contract Period : 12 months
Expected date of employment : 1 September 2022
Proportion of work : Full time
Remuneration : Bet. 2 130 and 2 260 gross monthly
Desired level of education : 5-year university degree
Experience required : 1 to 4 years
The mission will be integrated within the projects of the ReGArDS team, which is interested in understanding mechanims of gene expression regulation in human cells, with a particular focus on the impact of the chromatin structure on the processing of pre-messenger RNAs. (alternative splicing and polyadenylation).
- Carry out experiments of cell and molecular biology (maintenance and transfection of cell lines, extraction of RNA and proteins, analyses by RT-PCR/qPCR, Western-blot…), of molecular genetics (engineering of cells by CRISPR-Cas9 approaches) and of functional genomics (RNA-seq, ChIP-seq, CU&RUN-seq…)…
- Exploit and present the results of analyses, to guarantee their quality and follow-up.
- Write reports on experiments and protocols.
- Ensure that health and safety rules are respected.
- Ensure a scientific and technological follow-up in the domain or research.
- Participe to the diffusion and transfer of the results through oral presentations and publications.
- Accompany and train students (Master level); training to techniques used in the lab and to the daily functioning of the team.
Knowledge required :
- Master in molecular biology, cellular biology or genetics.
- A knowlegde in genomics and/or transcriptomics would be an advantage but is not a prerequisite.
- Routine techniques of molecular and cellular biology.
- Optimisation of experimental protocols.
- Organisation and analysis of data, knowledge of softwares for data management.
- Reporting and presenting results in team meetings.
- English level: B1-B2
- A knowledge in bioinformatic analyses would be an advantage (R).
- Rigour in performing experiments.
- Excellent organisation skills.
- Autonomy in the management of experiments.
- Good capacity of analytical and scientific reasoning.
- Easiness in relationships and team working.
ReGArDS lab (Regulation of Genome Architecture and Dynamics of Splicing), co-headed by Didier Auboeuf and Cyril Bourgeois, is located at LBMC (Laboratoire de Biologie et Modélisation de la Cellule) at the Ecole Normale Supérieure de Lyon. The team is currently composed of 3 permanent researchers, 4 bioinformaticians and 6 PhD students. The new engineer will be directly supervised by Cyril Bourgeois.
Constraints and risks
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