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Reference : UMR5239-ISASER-010
Workplace : LYON 07
Date of publication : Friday, January 31, 2020
Type of Contract : FTC Technical / Administrative
Contract Period : 17 months
Expected date of employment : 2 March 2020
Proportion of work : Full time
Remuneration : bet. 2 050 and 2 215 gross monthly
Desired level of education : 5-year university degree
Experience required : 1 to 4 years
The candidate will map DNA-RNA hybrid forming regions upon DNA replication stress using several yeasts as model systems.
We study the impact of gene transcription on genome stability. In some regions of the genome, transcription interferes with DNA replication by producing DNA-RNA hybrids. The position of such hybrids and their direct impact on DNA replication remain poorly understood however.
The candidate will optimize existing protocols to purify and sequence DNA-RNA hybrids and map such hybrids in several experimental setups.
We are looking for candidates with a strong training in cell biology and molecular biology.
Expertise in the handling of nucleic acids is also strongly desired.
Previous experience with the preparation of sequencing libraries would be a bonus.
"Laboratoire de Biologie et Modélisation Cellulaire" (LBMC) is a research institute located at the "Ecole Normale Supérieure de Lyon" in the Gerland neighborhood of Lyon and hosts fifteen research teams.
The institute aims at characterizing the molecular bases of cellular processes in normal and pathological conditions and develops genetic and quantitative analyses of biological systems and modelling approaches to do so.
The project will be carried out in collaboration with the teams of Philippe Pasero (IGH, Montpellier, France), Sarah Lambert (Institut Curie, Orsay, France) and Chunlong Chen (Institut Curie, Paris, France) as part of a recently funded ANR proposal (Project ReDeFine).
Constraints and risks
The candidate may have to spend short stays in the other teams of the research consortium.
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