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Reference : UMR5239-FABDUV-001
Workplace : LYON 07
Date of publication : Tuesday, November 10, 2020
Type of Contract : FTC Technical / Administrative
Contract Period : 36 months
Expected date of employment : 1 March 2021
Proportion of work : Full time
Remuneration : Monthly growth salary comprised between 2109 and 2228 € depending on experience.
Desired level of education : 5-year university degree
Experience required : 1 to 4 years
The collaborator will contribute to a research project aiming at understanding how new mutations influence the evolution of gene expression regulation. To this end, we will use innovative genomic approaches to characterize the transcriptomes of hundred of mutant and wild strains in yeast (Saccharomyces cerevisiae) grown with or without environmental stress. The main missions of the selected candidate will be to generate collections of mutant and wild type strains expressing RNA barcodes, to grow micro-colonies from encapsulated cells coming from these collections in presence or in absence of stress and to prepare cDNA libraries using an automated platform in order to quantify the transcriptome of thousands of samples via high-throughput sequencing.
The engineer will:
- Construct yeast strains obtained by chemical mutagenesis or by targeted mutagenesis using CRISPR/Cas9 for genome editing.
- Perform encapsulations and cultures of yeast cells in alginate microbeads and sort microcolonies in microplates using existing protocols.
- Adapt a protocol for extracting RNA and preparing cDNA libraries using a robotic platform.
- Present his/her work in laboratory meetings
- Participate (if possible) to workshops or conferences on the techniques/topics used.
- 2 years or more of experience as an engineer in molecular biology recommended
- Scientific and methodological rigor for setting up protocols
- Proficiency with basic molecular biology techniques absolutely required (cloning, PCR, nucleic acid extraction, gel electrophoresis…)
- Experience for preparing cDNA libraries or with other NGS techniques regarded
- Clear and precise writing of protocols and reports (English or French)
- Experience with genetic engineering in yeast regarded
- Understanding of RNA sequencing techniques
- Reading protocols and research articles in English
- Rigor and professionalism
The engineer will work under the supervision of F. Duveau (researcher) in the team “Genetics of intraspecies variation” led by G. Yvert (http://www.ens-lyon.fr/LBMC/gisv/index.php/en/) which is part of the Laboratoire de Biologie et Modélisation de la Cellule (Laboratory of Biology and Modeling of the Cell, UMR5239) at ENS de Lyon. She/he will benefit from the laboratory scientific environment (comprising 100 people distributed among 15 research teams), especially from the expertise of several teams on RNA sequencing techniques and from the access to shared platforms for flow cytometry, genomics and automated pipetting available at the LBMC.
The team is currently composed of a research director, a researcher, two engineers and a doctoral student (all affiliated with CNRS) and regularly hosts students for short-term internships. This hiring is covered by an ANR JCJC project starting in the upcoming year.
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