Post-doc (M/F) at LBMC, ENS-Lyon (France) - molecular genetics

Job Description

Missions

Our team is looking for a highly motivated postdoctoral researcher (M/F) to work on a project funded by the French National Research Agency (ANR). The project aims to understand the molecular function of DDX17 helicase in gene expression regulation and RNA processing. It builds on recent findings by the team, which identified genetic variants of the DDX17 gene associated with a neurodevelopmental syndrome and highlighted the role of DDX17 in coordinating certain epigenetic mechanisms and co-transcriptional RNA processing. To answer these questions, the project plans to use original cellular models to enable large-scale analysis of the functions of DDX17 and its variants (transcriptomics, genomics, interactomics), conventional approaches to protein and RNA molecular biology, and more targeted approaches to molecular epigenetics.

Activity

Experimental activities:
- Cell culture: cell culture and transfection
- Molecular biology: RNA and protein extraction, RT-PCR, qPCR, immunoprecipitation, Western blot analyses
- Molecular genetics and epigenetics (cell manipulation using CRISPR-Cas9 techniques)
- Functional genomics and transcriptomics (RNA-seq, ChIP-seq, etc.)

Related activities:
- Ensuring the application of health and safety principles and rules.
- Using and presenting analysis results, ensuring their follow-up and quality.
- Writing experiment reports and protocols.
- Keeping up to date with the project's bibliography.
- Participating in the dissemination and promotion of results in the form of oral presentations and publications.

Your Profil

Skills

Skills:
- In-depth knowledge of fundamental cell culture and molecular biology techniques.
- Good understanding of the general mechanisms regulating gene expression and RNA processing (alternative splicing, polyadenylation).
- Experience in the field of 'omics' approaches is desirable.
- Good command of English.

Personal skills:
- Rigorous, autonomous and strong ability to work in a team.
- Ability to synthesise information.
- Autonomous in planning and managing experiments.
- Analytical and scientific reasoning skills.
- Data organisation and analysis, use of data processing software.
- Reporting and presentation of results in team meetings.
- Good interpersonal skills and ability to work in a team.

Your Work Environment

The ReGArDS (Regulation of Genome Architecture and Dynamics of Splicing) team, co-led by Didier Auboeuf and Cyril Bourgeois, is based at the LBMC (Laboratory of Cell Biology and Modelling) at the Ecole Normale Supérieure de Lyon (France), which offers a stimulating and multidisciplinary environment conducive to a collaborative and collegial scientific atmosphere.
The team currently consists of three permanent researchers, several bioinformatics engineers and three doctoral students.
The successful candidate will report directly to Cyril Bourgeois.

Compensation and benefits

Compensation

Starting from 3071€ depending on experience (monthly gross salary)

Annual leave and RTT

44 jours

Remote Working practice and compensation

Pratique et indemnisation du TT

Transport

Prise en charge à 75% du coût et forfait mobilité durable jusqu’à 300€

About the offer

About the CNRS

The CNRS is a major player in fundamental research on a global scale. The CNRS is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to bring together different disciplines to address the most important challenges of the contemporary world, in connection with the actors of change.

CNRS

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