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Reference : UMR5237-CECGAU-002
Workplace : MONTPELLIER
Date of publication : Wednesday, September 02, 2020
Type of Contract : FTC Scientist
Contract Period : 36 months
Expected date of employment : 4 January 2021
Proportion of work : Full time
Remuneration : starting 2675 € brut
Desired level of education : PhD
Experience required : 1 to 4 years
We are looking for a postdoctoral researcher highly motivated by the field of cell biology and cancerology and with a particular interest in cell imaging in the context of vesicular trafficking.
General responsibilities include the design, implementation and interpretation of experiments, both independently and collaboratively. The candidate must communicate, in English and French, in a clear and concise manner his results.
Tumor cell invasion and metastasis formation are the main cause of death in patients with cancer. Exosomes that are secreted and exchanged between cancer cells and neighboring cells can favor migration, metastasis and a pro-tumorigenic micro-environment. They also represent a promising source of tumor growth biomarkers in body fluids.
We discovered that flotillin upregulation promotes the production of cadherin-bearing exosomes that could interfere with the inter-cellular adhesion properties. Flotillins are upregulated in many cancer types (carcinomas and sarcomas), and this is associated with poor prognosis and the acquisition of invasive cell properties.
(Gauthier-Rouvière et al, Cancer and Metastasis Review, 2020, doi: 10.1007 / s10555-020-09873-y).
The candidate's project aims to identify the molecular mechanisms by which overexpressed flotillins increase the secretion of exosomes containing N-cadherin.
This project combines the use of advanced cell biology and microscopy techniques (optogenetics, super-resolution and electron microscopy, imaging of living cells).
This project will be carried out in collaboration with two French teams, and will have a strong impact in the field of cancer cell biology and will open up new therapeutic perspectives for the diagnosis of aggressive tumors overexpressing flotillins.
The candidate should have:
- PhD in cell biology or biochemistry.
- Experience in cell culture, purification of exosomes, immunofluorescence, high resolution cell imaging, electron microscopy. Expertise in vesicular trafficking and / or lipid biology will be a plus.
- Scientific excellence attested by publications.
- Strong computer skills, including experience with image analysis, Prism and Excel.
- A high level of initiative, autonomy and the ability to assume responsibilities.
- Strong interpersonal and coaching skills are required to deal effectively with students and collaborations.
- Fluency in oral English and a strong ability to present results.
The project, funded by INCa for a period of 3 years, will be carried out in the team led by Cécile GAUTHIER-ROUVIERE (http://www.crbm.cnrs.fr/en/team/adhesion-and-vesicular- trafficking-in-tumoral-invasion /), and as part of a collaboration with the teams of Guillaume VAN NIEL (INSERM U1266 Paris, https://ipnp.paris5.inserm.fr/recherche/equipes-et-projets/ 17-equipe-van-niel) in order to study in vivo the impact of exosomes on inter-cellular adhesion, and Jessica GOBBO (INSERM Dijon, https://www.canceropole-est.org/la-recherche/ annuaire-du-canceropole-est / people / detail? id = 1207) in order to analyze whether the expression of flotillins affects the number and nature of exosomes, in relation to metastatic development.
The project will be carried out by the candidate at CRBM (http://www.crbm.cnrs.fr/en/), a laboratory equipped with an imaging platform (http: //www.mri.cnrs .fr / fr /) allowing the use of advanced microscopy techniques. The CRBM is located on the CNRS campus, bringing together around 500 researchers in biology, generating a site rich in scientific and technological interactions.
Publications related to the project
- Gauthier-Rouvière C, Bodin S, Comunale F and Planchon D. (2020) Flotillin membrane domains in cancer. Cancer and Metastasis Review 2020. doi: 10.1007/s10555-020-09873-y
- Planchon D, Morris E, Genest M, Comunale F, Vacher S, Bièche I, Denisov E, Tashireva L, Perelmuter V, Linder S, Chavrier P, Bodin S*, Gauthier-Rouvière C*. (2018) MT1-MMP targeting to endolysosomes is mediated by flotillin upregulation, Journal of Cell Science, 131. doi: 10.1242/jcs.218925
Research highlight, J Cell Sci e1703. * Equal last authors.
- Morris E, Bodin S, Delaval B, Comunale F, Georget V, Costa ML, Lutfalla G, Gauthier-Rouvière C. (2017) Flotillins control zebrafish epiboly through their role in cadherin-mediated cell-cell adhesion. Biology of the Cell, 109:210-221. doi: 10.1111/boc.201700001
- Bodin S, Planchon D, Rios Morris E, Comunale F and Gauthier-Rouvière C. (2014) Flotillins in intercellular adhesion: from cellular physiology to human diseases, J of Cell Sci, , 127, 5139-5147. doi: 10.1242/jcs.159764
- Guillaume E, Comunale F, Do Khoa N, Planchon D, Bodin S and Gauthier-Rouvière C. (2013) Flotillin micro-domains stabilize Cadherins at cell-cell junctions. Journal of Cell Science, , 126, 5293-5304. doi: 10.1242/jcs.133975
Highlighted in "http://f1000.com/prime/718114375?bd=1&ui=154605"
Constraints and risks
Biological risk (P2)
The candidate must provide a CV, a cover letter and two letters of reference.
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