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Reference : UMR5237-ALECOP-002
Workplace : MONTPELLIER
Date of publication : Wednesday, April 14, 2021
Type of Contract : FTC Technical / Administrative
Contract Period : 12 months
Expected date of employment : 1 June 2021
Proportion of work : Full time
Remuneration : 2110,00 monthly salary
Desired level of education : 5-year university degree
Experience required : Indifferent
Develop robust protocols for the analysis of protein localization and dynamics in different cell models (yeast, 2D cell culture and spheroid cultures) by confocal and super-resolution microscopy. Development and use of real-time fluorescence assays in microfluidic chambers. Construction of fluorescent proteins and preparation of cell samples for fluorescent imaging.
1. Perform fluorescent imaging of live or fixed cell samples (yeast, 2D and 3D spheroid cultures of mammalian cells)
2. Quantitatively analyze images and develop automated image analysis scripts
3. Analyze protein and DNA sequences using bioinformatics tools
4. Build plasmids for fluorescent protein expression using molecular biology
5. Prepare live or fixed cell samples for fluorescent imaging, cell culture, etc.
- Excellent knowledge of confocal fluorescence microscopy, be familiar with super-resolution techniques
- Good knowledge of bioinformatics tools applied to molecular biology, biochemistry, microscopy and statistical analysis
- Image analysis in ImageJ
- Technical and scientific monitoring of the literature
- Good presentation and scientific communication skills; ability to communicate in English
- Good knowledge of molecular and cellular biology
- Punctuality, autonomy, teamwork
The Cell Biology Research Center of Montpellier (UMR 5237 between CNRS and the University of Montpellier) is organized in 16 research teams, technological platforms and common services, and counts about 150 people (researchers and teacher-researchers, engineers, technicians and administrative staff, PhD and post-doctoral students). The CRBM is connected with other institutes on the same campus where it shares different platforms, including the MRI imaging platform equipped with the most advanced microscopes and dedicated staff.
The engineer will integrate in the team of Alenka Copic "Lipid storage and transfer in the cell", which is currently under construction and includes a postdoctoral researcher. We use different cellular and biochemical models to understand how proteins control the storage and distribution of lipids in eukaryotic cells.
The work is part of an ERC - synergy research program entitled 'Spheres' uniting several research teams in France and Sweden. The objective is to understand the mechanisms controlling the size of lipid droplets in adipocytes.
Constraints and risks
Expected travel to France and Sweden, the engineer (M /F) will interact regularly with other members of program synergy.
Duration of the contract: 1 year renewable up to 3 years
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