Missions

To create transgenic Drosophila melanogaster lines for fluorescence imaging experiments for tracking the sub-nuclear distribution of transcription factors, developmental genes, and epigenetic marks inside embryos.

Activités

- Design and generate transgenic Drosophila melanogaster lines with developmental genes or transcription factors tagged for fluorescence microscopy
- Design and optimize protocols to prepare fly embryos for fixed and live fluorescence imaging at high- or super-resolution
- Manage and maintain transgenic fly lines
- Manage laboratory operations related to the above-named activities

Compétences

The candidate must be highly motivated and autonomous and have experience working with laboratory animals and their genetics, ideally with Drosophila. Prior experience with fluorescence imaging (confocal or widefield) is highly beneficial. The candidate must have sufficient command of English to communicate professionally and to understand technical documentation and scientific literature (B2 level).

Contexte de travail

The Centre de Recherche en Biologie cellulaire de Montpellier (CRBM) UMR 5237 is located in the CNRS campus, which also hosts the IGMM, the IRIM, and the Pôle Chimie Balard. The CRBM consists of 160 researchers, post-docs, doctoral students, and technical staff, covering a wide range of topics in cellular biology. It has access to the MRI, a state-of-the-art imaging platform, and to the Drosophila platform at the IGH, the largest fly center in France. The recruited person will work in the TSAI Group (Nuclear organization of transcription in embryos) to perform the activities related to generating transgenic fly lines and optimizing protocols for microscopy experiments.

Contraintes et risques

No particular security conditions.