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Reference : UMR5235-MAINGU-003
Workplace : MONTPELLIER
Date of publication : Monday, March 09, 2020
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 4 May 2020
Proportion of work : Full time
Remuneration : Salary between 2695 and 3840 euros according to the experience of the candidate
Desired level of education : PhD
Experience required : 1 to 4 years
The young scientist will participate to the design of the experiments and will perform these experiments which include live imaging in the zebrafish larva, preparing samples for single cell RNA-seq. He/She will also analysis the data.
The project combine several approaches in genetic, high resolution imaging and transcriptomic analysis.
The candidate will:
-participate to the design of the project
-perform experiments and analyses the data
-Communicate the results
-Participate to the writing of the publications
The candidate should have an expertise in the zebrafish model and/or in the field of immunology. Knowledge in the CrispR/Cas9 system would be appreciated.
The candidate should harbor excellent skills in the organization of his/her work and in interacting with a team. He/She should be able to speak and write in English language.
Research at Laboratory of Pathogen Host Interaction (LPHI) focuses on the study of host-pathogen interactions, with emphasis on infections by parasites (Plasmodium, Toxoplasma), bacteria (Mycobacterium, Staphylococcus, Salmonella) and viruses (virus particle biogenesis and antiviral defenses). Three main axes are developed in the department : parasitology, microbiology and immunology. While some groups are using mammalian systems, the zebrafish is widely used at LPHI.
The team in which the candidate will be recruited use the zebrafish as a model system to studying innate host defense mechanisms involved in bacterial infections and innate immune responses during wound healing. The optical transparency of the zebrafish embryo combined with innovative technics in cell labelling provides an outstanding opportunity to visualize innate immune cells including neutrophils and macrophages during an infection or a tissue injury. In this context we study how the phagocytes respond to environmental signals and change their fate. This project financed by the Agence National pour la Recherche (ANR) will be more particularly focused on the dynamics of macrophage polarization during wound healing.
The group has acquired an international renown in the generation of transgenic lines that are instrumental for immune studies and that have been asked by laboratories all over the world.
The candidate will be supervised by Mai Nguyen-Chi in the team of Georges Lutfalla « Onset of immunity and inflammation ». He/She will have access to the zebrafish facility of the university of Montpellier and all the innovative techniques in microscopy and genimoc analysis thanks to the platforms of Montpellier, MRI and MGX.
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