Faites connaître cette offre !
Reference : UMR5203-GUILEB-002
Workplace : MONTPELLIER
Date of publication : Wednesday, February 17, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 March 2021
Proportion of work : Full time
Remuneration : from 2400 and 2700 € growth wages.per month
Desired level of education : PhD
Experience required : 1 to 4 years
The selected candidate will developed a research project that aim at deciphering the activation mechanism of G protein coupled receptors (GPCRs). The candidate will express and purify GPCR and GPCRs signalling complexes and performed biophysical and structural characterisation of the purified receptor. The main objective is performed structural characterisation of GPCR signalling complexes at the atomic scale.
-Molecular biology and protein engineering (mutagenesis, unnatural amino acid labelling)
-Membrane protein expression and purification
-sample reconstitution in membrane-mimicking environment (nanodisc)
-Biochemical and pharmacological characterisation of the signalling complexes.
-Sample preparation for cryoEM
- Molecular Biology
- Cell culture,
- Large scale expression and purification of membrane proteins.
- Membrane protein biochemistry.
- CryoEM grid freezing
The IGF is University, CNRS and INSERM research unit located on the Medical Sciences campus in Montpellier (http://www.igf.cnrs.fr). IGF 19 research teams develop multidisciplinary research project from the atomic scale to animal model. Our institute is internationally established in the field of GPCRs and molecular pharmacology and provide a state of the art research environment.
The selected candidate will be part of the team Molecular and structural mechanism of neuromodulation, in the neuroscience department.
We are seeking a highly motivated biochemist with experience in molecular biology, membrane protein expression (insect and mammalian cells) and purification. Experience with cryoEM would also be appreciated but not essential, as training will be provided.
Please apply online.
Constraints and risks
We talk about it on Twitter!