Reference : UMR5168-LAEKUR-001
Nombre de Postes : 1
Workplace : GRENOBLE
Date of publication : Friday, January 20, 2023
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 April 2023
Proportion of work : Full time
Remuneration : 2800 E-3960 gross salary per month
Desired level of education : PhD
Experience required : 1 to 4 years
Understanding what regulates cell size is a fundamental question in biology. Many organelles and subcellular structures established during development can indeed maintain their size over cell growth or shape change. Among those, the actin cytoskeleton can form different stable while dynamic intracellular architectures that play a major role in the structural plasticity of cells in response to such changes. However, the mechanisms of scaling of the actin networks with cell size are still poorly understood. In particular, how the cells adapt the turnover and organization of their numerous structures with cell size remains unknown. The selected candidate will thus study the scaling of actin structures with cell size.
The candidate will carry out his postdoctoral work in Grenoble, where he will be in charge of developing a cell assay to characterize the organization, size and dynamics of actin structures according to a variation in cell size. He/she will first have to read the bibliography associated with the scaling of structures/organelles with the size of cells. In parallel, he will also have to gain hand on expertise on the different techniques available in the laboratory that will allow him/her to # control the geometry and organization of cells (micropatterning) # to mark the actin structures (choice of the type of actin construct and cell line used) # and to characterize them (fluorescence microscopy imaging and development of an associated analytical pipeline). Finally, the selected candidate will be expected to benefit from technological and methodological developments made in the laboratory (cell enucleations, cell fusions, microinjection, strecthnig, etc.) to possibly modulate the organization of cell architectures or biochemical conditions of the test.
The candidate will have to possess a strong background in cell biology and microscopy imaging. He will be expected to learn the cutting-edge techniques available in the lab (micropatterning, microinjection, cell enucleation...) to set up an original cellular assay allowing a better understanding of actin dynamics.
Cytomorpholab is a team of about 20 people co-directed by Laurent Blanchoin and Manuel Théry. It is located on two sites: Grenoble (where the postdoc will take place) and Paris. It is part of the PCV laboratory (Laboratoire de Physiologie Cellulaire et Végétale; UMR 5168 CEA-CNRS-UNIV. GRENOBLE ALPES – UMR 1417 INRA). At the scientific level, the Cytomorpholab has developed a unique and internationally recognized expertise in the study of cytoskeleton through complementary in vitro and cellular approaches. At the cellular level, by combining micropatterning techniques with laser photoablation and microscopy of traction forces, the laboratory's work has demonstrated the mechanical impact of stress fibres and the surrounding cortical network in the magnitude of forces produced by cells (Vignaud T., Nat Mat, 2021). Our laboratory has also recently used tubulin microinjection to demonstrate the self-repair of microtubules in living cells (Gazzola M., Current Biol, 2023). Finally, by combining the use of enucleated cells with micropatterns, the team also highlighted a key role of the geometry of the actomyosin network in the positioning of the centrosome (Jimenez AJ., Current Biol, 2021). The candidate will thus evolve in a dynamic and stimulating environment where he will have access to advanced techniques (microscopy, microfabrication, biophysics etc.).
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