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Reference : UMR5168-CHLZUB-001
Workplace : GRENOBLE
Date of publication : Thursday, January 09, 2020
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 April 2020
Proportion of work : Full time
Remuneration : gross monthly salary between 2617-3729 depending on experience
Desired level of education : PhD
Experience required : 1 to 4 years
The postdoctoral researcher will be part of the Integrated Structural Biology and Plant Development team of the Laboratoire de Physiologie Cellulaire et Végétale in Grenoble. The postdoctoral researcher will be responsible for designing experiments to biophysically and structurally characterize plant proteins and protein complexes (EARLY FLOWERING 3 and the Evening Complex) important for thermoresponse in Arabidopsis. The postdoctoral researcher will develop expression and purification protocols for recombinant protein production, characterize the proteins individually and in complex by crystallography, SAXS, NMR and/or electron microscopy and analyze their activity as a function of temperature in vitro and, ideally, in vivo. In addition, the postdoctoral researcher will aid in supervising students/technicians. The researcher will be located and perform his/her experiments at the CEA and ESRF campuses. The postdoctoral researcher will be supervised by C. Zubieta.
The postdoctoral researcher will develop protocols for the expression and purification of proteins important for thermosensing and thermoresponse in Arabidopsis. This requires recombinant expression tests in prokaryotic and eukaryotic expression systems and familiarity with these techniques are required. The postdoctoral researcher will perform crystallization screening, data collection and structure solution. Structural experiments, including protein crystallography, small angle scattering, NMR and/or EM, will be implemented and performed by the postdoctoral researcher in collaboration with team members and on-site collaborators. In addition, different biochemical and biophysical techniques including circular dichroism, thermal shift assays and optical and fluorescence microscopy will be performed to characterize the structure of the proteins of interest as a function of temperature. The postdoctoral researcher will help supervise thesis and Master's students, contribute to the redaction of manuscripts and present results at national and international conferences. The results obtained will contribute to our understanding of plant thermosensing and response. These results will have potential long-term applications to the field of bioengineering of desired traits in crop species.
Required skills include experience in molecular biology (design of different constructs, mutants, etc), protein biochemistry (protein expression, purification and characterization) and structural biology (crystallography, SAXS, NMR and/or EM experience is required). The candidate will have a doctorate in structural biology or a related field. The candidate must be fluent in spoken and written English and ideally possess a working knowledge of French. Candidates must have at least one first or second author publication in structural biology or biophysics. Candidates must be able to work collaboratively in a team.
The candidate will work jointly at the ESRF molecular biology laboratory as part of the structural biology group and the Laboratoire de Physiologie Cellulaire et Végétale (UMR 5168) at the CEA. The candidate will have access to the beamlines and EM facilities of the ESRF and the NMR facilities of the Institut de Biologie Structurale (IBS) via established collaborations. Microscopy experiments will be performed at the CEA and uLife platform. The resources necessary for the realization of the project are located on the ESRF and CEA campuses in Grenoble, all of which the candidate will have access to during the course of the project.
Constraints and risks
The candidate will be required to travel between the ESRF and CEA campuses depending on the experiments to be performed. The campuses are located adjacent to each other in Grenoble and are accessible by tram, bike or on foot.
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