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Reference : UMR5099-KERBYS-002
Workplace : TOULOUSE
Date of publication : Tuesday, September 15, 2020
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 4 January 2021
Proportion of work : Full time
Remuneration : 2600-3000 EUR/m
Desired level of education : PhD
Experience required : Indifferent
The role of genome dynamics and structure in the regulation of transcription activation.
- Live cell imaging, use of new developments (single locus tracking, whole genome imaging)
- quantitative image analysis, use and optimisation of algorithms
- live cell imaging
- quantitative image analysis, programming a plus
- cell culture a plus
- at ease with designing eperiments, scientific writing, presentations
To dissect the mechanisms by which chromatin structure and dynamics regulate gene expression, motion of DNA and proteins have to be analysed in real time and single cells. We have studied architectural changes in chromatin of key genes sensitive to hormone in mammary tumor cells (MCF7) at different scales and have shown that the movement of chromatin is constraint during the initiation of transcription (Germier, Biophys. J, 2017; Shaban bioRxiv 2018). The transcription factor, the estrogen receptor α (ERα), is known to form foci in cells treated with a hormone. Our goal is to show if these foci represent transcription factories that can form around chromatin fibers and loops and if mRNA output is dependent on this dynamic.
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