Missions

The postdoctoral researcher will work on developing in vitro and in vivo approaches aimed at deciphering the metabolic vulnerabilities of brain cancer cells' adaptation to chemotherapy. The work will be conducted as part of a competitive funding obtained by a consortium of French teams, which have complementary models, tools, and expertise in neuro-oncology, including cancer cell metabolism and computational biology.

Activities

Main Activities
• Implementing experimental assays required for the project (genetic and pharmacological manipulation of human cancer cells, cellular biology assays, biochemistry, metabolic profiling, measurement of metabolic activities, in vitro imaging using time-lapse and confocal microscopy, light-sheet imaging, and bioluminescence imaging).
• Monitoring relevant literature for the project.
• Presenting results during weekly lab meetings and at national and international scientific conferences.
Secondary Activities
• Interacting with other team members and collaborators.
• Contributing to in silico modeling of metabolic deregulations.
• Participating in in vivo experiments (access to immunodeficient animals and neurosurgery).
• Participating in the lab's collective activities (e.g., meetings, seminars).

Skills

Scientific skills :
• Strong skills in chemistry, cell and molecular biology and in metabolic analysis.
• Knowledge of neuro-oncology.
• Level 1 animal experimentation required (mouse handling, force-feeding, surgery).
• Knowledge regarding the functioning of cerebral metabolic systems or glioblastoma will be appreciated.
• Language skills: English reading spoken essential in the international group.

Know how :
• Good skills in human cell culture, microscopy, good knowledge of metabolic flows, metabolomic and transcriptomic analyses.
• Quickly adaptation to new methodologies.
• Know how to use basic IT tools and software dedicated to data processing.
• The postdoctoral fellow must be motivated, rigorous, endowed with a good sense of organization and capable of autonomy.
Other training courses intended to enrich the knowledge of the postdoctoral fellow may be offered during the duration of his or her CDD.

Work Context

The post-doctoral fellow will work within the framework of obtaining competitive funding obtained from the INCA, with the call for PAIR 2022. This scientific consortium of high-performance laboratories includes teams based on University of Bordeaux (CNRS) and Sorbonne University (Paris). Thomas Daubon's group (IBGC UMR5095) brings together an Associate Professor, 1 permanent Researcher, 3 postdoctoral fellows, 5 doctoral students, master's students, 2 engineers.
Thomas Daubon has many local, national, but also international collaborations, which will be so many assets for the success of this project.
This contract is for 18 months.

Constraints and risks

Schedules will be made within regulatory working hours. The postdoctoral scientist must go to several sites to perform the experiments (vehicle needed). The candidate will work on immunocompetent mouse models, with patient samples. The candidate will work on immunodeficient and immunocompetent mouse models, with patient samples. However, all health risks are perfectly controlled by the team. The candidate will be trained in the potential risks associated with the project by the manager upon arrival at the laboratory. He / She will also receive training for new entrants provided by the Prevention Assistants of the Unit.