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Reference : UMR5095-ISANIC-010
Workplace : BORDEAUX
Date of publication : Wednesday, November 17, 2021
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 February 2022
Proportion of work : Full time
Remuneration : Between 2663 and 3783 € gross monthly according to experience
Desired level of education : PhD
Experience required : 1 to 4 years
A post-doc position funded by the Région Nouvelle Aquitaine is open in the team of Dr. Damien Coudreuse at the Institut de Biochimie et Génétique Cellulaires (IBGC, CNRS UMR 5095, Bordeaux, France). Research in the team focuses on different aspects of eukaryotic cell proliferation, aging and evolution. The proposed project aims at studying the role of cell volume, cytoplasmic fluidity and macromolecular crowding in the process of chronological aging.
For this project, the recruited person will use a simple model system, the fission yeast Schizosaccharomyces pombe. The proposed work will take an interdisciplinary approach, combining state-of-the-art methods in genetic engineering, live-cell imaging, microfluidics and analysis of physical parameters.
The candidate will be responsible to lead the project with help from the group leader. She/he will develop the scientific strategy, perform the appropriate experiments, interpret the results and propose new approaches to fully develop the project. Her/his activities will include (but are not restricted to):
- Design and fabrication of microfluidic chips by photolithography.
- Perform live-cell imaging assays by confocal microscopy (multi-color time-lapses) coupled with microfluidics.
- Analyze the microscopy images using various softwares.
- Generate the yeast strains that are necessary for the project, including strains that will allow for the monitoring of cytoplasmic and nuclear fluidity.
- Take advantage of her/his expertise in biophysics to introduce new technologies and analysis strategies.
- Present her/his results during lab meeting, in the context of internal seminars as well as at national and international scientific conferences.
- Contribute to the writing of scientific manuscripts presenting the data obtained in the context of the project.
- Participate to all common tasks of the team.
Importantly, the candidate will adapt her/his activities to the progression of the project and be trained in new techniques when necessary.
The candidate must have a solid background in cell biology and biophysics. She/he should also have significant prior experience in microfabrication (soft lithography), in the use of microfluidic devices for live-cell imaging and in microscopy. The applicant should also possess expertise in the quantitative analysis of biological parameters, in image analysis as well as good notions of coding (Python, R). Candidates with prior experience in the study of the mechanisms that control cell morphology and physical features such as size and internal fluidity are encouraged to apply. A very good level of spoken and written English is essential as all team meetings and discussions are in English.
The two first months of the contract will be held at the Institute of Genetics and Development of Rennes (UMR 6290, Rennes). The team will then be located, for the remainder of the contract, at the IBGC (UMR 5095, Bordeaux). The IBGC is the main research center for fundamental cell biology in Bordeaux. Research at the Institute covers a large spectrum of areas, including studies of cell proliferation, cellular quiescence, cytoskeleton dynamics, chromosome biology, and cell metabolism. With 12 research teams and about 100 members, the IBGC offer a dynamic and collegial scientific environment. The language used in the team is English.
The IBGC is located in Bordeaux, a very welcoming and pleasant city with a rich cultural tradition. Bordeaux can be rapidly reached from Paris (2h by train) and has a well-connected international airport.
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