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Reference : UMR5089-OLINEY-002
Workplace : TOULOUSE
Date of publication : Monday, November 02, 2020
Type of Contract : FTC Scientist
Contract Period : 36 months
Expected date of employment : 4 January 2021
Proportion of work : Full time
Remuneration : Between 2648 and 3054 € gross monthly depending on experience
Desired level of education : PhD
Experience required : Indifferent
We are seeking for a highly motivated postdoctoral fellow, with a particular interest in infection biology, including microbiology and immunology. The employment is for 3 years, aiming to better understand host-pathogen interactions in tuberculosis. General responsibilities include design, implement and interpret experiments, both independently and in collaboration, and communicate research and findings in a clear and concise manner.
With 1.5 million deaths due to tuberculosis (TB) in 2018, Mycobacterium tuberculosis (Mtb), the agent of TB claims more lives than any other single pathogen. Novel antibiotics, a vaccine better than BCG and alternative therapies are urgently needed to combat TB. This project aims at exploring the dynamics of host-pathogen interactions in TB using unprecedented, curving-edge technologies in vivo, at the level of the TB lung lesion.
Using a combination of reporter in vivo models, comics approaches, and high-end imaging, the project will:
- identité and characterize Mtb genes and pathways required for Mtb survival within different host microenvironment such as hypoxic, necrotic or caveating lesions. Microdissected lesions will be analyzed using various omics approaches, including transcriptomics and lipidomics.
- Establish the origins of necrotic and caveating lesions and the dynamics and function of immune cells in these different microenvironment during infection (cell recruitment, cell positioning). Individual lesion progression will be established using light-sheet and intravital microscopy. Specific depletion of immune cells will be used to determine their role in progression of specific lesions.
• A PhD degree preferably in infection biology, with experience in the use of in vivo models of infection.
• Scientific excellence evidenced by publication track record.
• Hands-on experience of in vivo experiments. Expertise in imaging will be prioritized. Expertise in molecular biology will also be considered.
• Experience in working in BSL-3 facilities.
• Strong computer literacy including experience with image analysis, FlowJo, Prism, and Excel.
• High levels of initiative, autonomy and the ability to assume a high level of responsibility.
• Strong interpersonal and mentoring skills needed to effectively deal with students and people of collaborating groups.
• Proficiency in English is a plus.
Additional qualifications desired
• Experience of cell culture of mammalian primary cells, and basic immunology assays such as multi-color flow cytometry, immunofluorescence and ELISA.
• Knowledge of histology, quantitative PCR, and general lab protocols and methodologies used in the biological sciences.
The project is a collaboration between three teams at IPBS (http://www.ipbs.fr), Toulouse (France), and will involve strong connection with the IPBS imaging platform, functional exploration platform; it will also involve collaboration with other services in Toulouse (laser microdissection, omics technologies). The state-of-the-art imaging technology in the Biological Safety Level 3 (BSL-3) facilities at IPBS and the combination of expertise between the teams assure the successful outcome of this project.
Constraints and risks
Work in BSL-3 environment.
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