Missions

The project aims to implement proton-detected fast MAS solid-state NMR methods to investigate the structure of viral membrane proteins and their interactions with nucleic acids. A central objective is to implement and test innovative proton polarization transfer approaches to characterize intermolecular interactions between macromolecules.
The protein solid-state NMR team focuses on the study of virulence factors in emerging infectious diseases, with the goal of better understanding molecular mechanisms of infection. Our current work centers on Buniaviricetes, three of which are listed among the World Health Organization (WHO) top-ten priority pathogens, including the highly deadly Crimean-Congo hemorrhagic fever virus (CCHFV).
The successful candidate will primarily focus on NMR methodology, comprising three major points: the full use of the selective labeling potential of cell-free protein synthesis, in the context of combinatorial labeling approaches for sequential assignments; the introduction of new isotopes into protein NMR; and the optimal implementation of intermolecular polarization transfer schemes. S/he will also produce samples following well-established protocols, mainly based on cell-free synthesis, but also bacterial expression. S/he will analyze them by solid-state NMR, potentially complemented by other biophysical approaches and solution NMR. The candidate will work closely with other team members involved in complementary aspects of this larger project.
The project is supported by outstanding funding over several years (https://www.fondationbs.org/notre-communaute/laureats-et-projets/anja-bockmann). More information about the team's research projects can be found on the group webpage: https://mmsb.cnrs.fr/en/team/protein-solid-state-nmr/.
The position is available starting January 2026, with later being possible. The position is initially funded for one year, with possible extensions up to three years.

Activities

- NMR measurements (0.7 mm 1H-detected MAS NMR) and implementation of advanced approaches
- NMR spectra analysis (CCPN) and data interpretation
- Protein preparation using cell-free/bacterial protein synthesis, design of dedicated labeling variants
- Biophysical characterization of the proteins and their interactions

Skills

The ideal candidate holds a PhD in chemistry, biochemistry or biophysics (or related discipline), and a strong interest in interdisciplinary work on (viral) proteins. S/he will have experience in solution- and/or solid-state NMR spectroscopy.

Work Context

The protein solid-state NMR laboratory is located within the Molecular Microbiology and Structural Biochemistry (MMSB) unit in the Lyon Gerland Bioscience Campus, regrouping major institutions in virology such as the CIRI and the Lyon BSL4 lab. The campus is located in close proximity to the city center and with convenient access to public transportation, including the subway, tram, and cycle paths. The lab has extensive spectrometer time at the High Field NMR Center of Lyon (CRMN), on several NMR spectrometers (500 MHz, 600 MHz, 800 MHz and 1000 MHz) and fast-MAS probes (2 x 0.7 mm probes, 1.3 mm probe, …), and higher fields at national facilities and through collaboration. The MMSB is fully equipped with a wet lab for protein expression and purification. Access to a range of biophysical platforms (ITC, SEC-MALLS, nanoDSF, mass spectrometry …) is given at the MMSB. We offer a welcoming environment with a friendly and satisfying working atmosphere.

The position is located in a sector under the protection of scientific and technical potential (PPST), and therefore requires, in accordance with the regulations, that your arrival is authorized by the competent authority of the MESR.