Missions

We are currently seeking a postdoctoral fellow (PD) in the field of biomolecular solid-state NMR to join our team at the CNRS/Lyon University in France (https://mmsb.cnrs.fr/equipe/rmn-du-solide-des-proteines/).

The project is situated within the broader context of developing strategies to more effectively prevent and control the emergence of infectious diseases, within the framework of a larger consortium. Bunyaviruses represent several of the priority pathogens listed by the World Health Organization (WHO) as possible causes of future pandemics, including Crimean-Congo hemorrhagic fever virus (CCHFV), Lassa fever virus (LASV), and Rift Valley fever virus (RVFV). Several of these viruses currently emerge in southern parts of Europe. In this context, our objective is to contribute a molecular understanding of the mechanisms of infection.

The objective of the PD is to examine the structures and interactions between the membrane-bound proteins of these viruses. To achieve this, the PD will utilize mainly proton-detected solid-state NMR spectroscopy, but also other biophysical approaches, to examine the samples she/he prepared using biochemical techniques that are well-established in the lab. Depending on the candidate's specific interests, the development or implementation of innovative NMR approaches to analyze the specific characteristics of proteins, or the design of novel biochemical approaches (labeling schemes, use of isotopes) are strongly encouraged. The PD will work closely with other lab members involved in different aspects of this larger project.

Activities

- Protein preparation using cell-free protein synthesis, design of dedicated labeling variants
- Biophysical characterization of the proteins and their interactions
- NMR measurements (0.7 mm 1H-detected MAS NMR), implementation of innovative approaches
- NMR spectra analysis (CCPNmr)
- Data interpretation
- Promotion of results through publications and participation in conferences
- Drafting of reports, technical documents and scientific publications

Skills

We are looking for highly motivated, talented and ambitious young scientists to join our collaborative and enthusiastic team. The successful candidate should have a degree in structural biology/biochemistry/biophysics and should be proficient in protein NMR, liquid or solid-state, and NMR data analysis. Experience in biochemistry is a plus. The position is initially a one-year contract, with the possibility for renewals. Salary is in accordance with CNRS regulations and commensurate with experience and post-thesis annuities. The position will commence at the earliest in January 2025.

Work Context

Research will be carried out at the Molecular Microbiology and Structural Biochemistry institute in the Lyon Gerland Bioscience Campus, next to the Center for Research in Infectiology and the Jean Mérieux lab. MMSB is a multi-disciplinary Joint Research Unit (UMR) managed, in addition to the CNRS, by the University of Lyon. The aim at MMSB is to understand the molecular mechanisms underlying microbial pathogenesis, and to study molecular and macromolecular interactions in structural, dynamic and functional terms. Groups there use cellular biology and biochemistry approaches in combination with structural biology. MMSB hosts 11 different research groups, totaling > 50 members. The Institute is in close proximity to the city center and with convenient access to public transportation, including the subway, tram, and cycle paths. The laboratory offers a welcoming environment with a friendly and satisfying working atmosphere, excellent facilities, as well as training and development opportunities. As the second largest city in France, Lyon is a prominent center for research on viral disease, and the project is anchored in a collaborative network including virologists and structural biologists from different horizons. The MMSB has access to a range of high-field NMR instruments, including those at the CRMN Lyon (500-1000 MHz) and national platforms (1.2 GHz). The spectrometers are equipped with 0.7 mm MAS probes.

The position is located in a sector under the protection of scientific and technical potential (PPST), and therefore requires, in accordance with the regulations, that your arrival is authorized by the competent authority of the MESR.

Constraints and risks

NA