Missions

A 10-months, non-renewable, postdoctoral fellow position is available for a project to be executed jointly in the “Cell Cycle & Cancer” team at the Centre for Integrative Biology/MCD UMR5077, (https://cbi-toulouse.fr/eng/equipe-besson) and ELIA team in the Laboratoire d'Analyse et d'Architecture des Systèmes (LAAS-CNRS) (www.laas.fr) in Toulouse, France.
The project aims at developing 3D microphysiological systems recreating the intestinal architecture and environment with the goal of studying intestinal stem cell fate and understanding the mechanisms leading to the development of cancers. 3D bioprinting and microfluidics methods will be used for the development of scaffolds mimicking the architecture of the intestinal epithelium and to recreate biochemical gradients and mechanical forces present in the intestine. These 3D microphysiological systems will be populated from primary intestinal cells, intestinal organoid-derived cells or iPSC-derived cells directed to differentiate in intestinal lineages.

Activités

The selected candidates will:
-actively participate in the conception of the project
-use and develop methodologies according to the need of the project
-perform experiments
-analyze and present data
-participate in writing research and review articles
-present the results
The candidate will use a broad panel of approaches in cell biology (imaging, cytometry, tissue culture, intestinal organoid and iPSC cultures) and molecular biology (cloning…) on primary cells and cell lines, as well as micro/nanotechnology (3D Bio impression, microfluidics, AFM…).

Compétences

Candidates (M/F) must hold a PhD and have strong skills in molecular and cellular biology and biophysics. Prior experience with intestinal organoids, stem cells and iPSC cultures and of 3D impression techniques and microfluidics is required. The candidate must have a strong publication record, be rigorous, and have strong organizational and teamwork skills. The candidate must be able to communicate in English (written/oral), knowledge of French is not mandatory.

Contexte de travail

Founded in 2016, the Centre for Integrative Biology of Toulouse (CBI; http://cbi-toulouse.fr/eng/) is an institute regrouping three research departments in Biological Sciences of the University of Toulouse and the French National Centre for Scientific Research (CNRS). It is located in Toulouse, South West of France, in the main Campus of the Université Toulouse III-Paul Sabatier (http://www.univ-tlse3.fr/), which offers a multidisciplinary education in the fields of science, health, engineering, and technology, developing one of the most important scientific research clusters in France.
The CBI currently brings together in a brand new research building more than 400 scientists in 40 research teams with worldwide recognition, aimed to foster research leading to the elucidation of fundamental aspects of the structure and function of complex biological systems, and covering all scales from individual molecules to the whole organism and population.
The LAAS CNRS (UPR8001) conducts research activities at the intersection of materials engineering, applied physics and life sciences. The main objective of these interdisciplinary activities is to develop micro- and nano-systems dedicated to applications in the fields of biology and environment. In these fields, the main axes of development are the design and integration of sensors, the development of microfluidic devices for analysis and diagnosis, the optimization of 2D/3D structuring technologies for materials and biomaterials, the realization of model devices for cellular engineering (pathophysiological context) and the biophysical exploration of tissues (detection based on mechanical characterization, electrochemistry, electromagnetism and optics).

Le poste se situe dans un secteur relevant de la protection du potentiel scientifique et technique (PPST), et nécessite donc, conformément à la réglementation, que votre arrivée soit autorisée par l'autorité compétente du MESR.

Contraintes et risques

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Informations complémentaires

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